An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon
文献类型：期刊论文
| 作者 | Wu, Hongyu; Xue, Xiaodong; Qin, Caihua; Xu, Yi; Guo, Yuyu; Li, Xiang; Lv, Wei; Li, Qinxia; Mao, Chuangxue; Li, Luzhao |
| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 180期号:1页码:56-65 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.18.00875 |
| 文献子类 | Article |
| 英文摘要 | Transposon tagging is a powerful tool that has been widely applied in several species for insertional mutagenesis in plants. Several efforts have aimed to create transfer-DNA (T-DNA) insertional mutant populations in Brachypodium distachyon, a monocot plant used as a model system to study temperate cereals, but there has been a lack of research aimed at using transposon strategies. Here, we describe the application of a maize (Zea mays) Dissociation (Ds) transposon tagging system in B. distachyon. The 35S::AcTPase cassette and Ds element were constructed within the same T-DNA and transformed into B. distachyon plants. The Ds element was readily transposed to other chromosomes or to the same chromosome under the function of Activator (Ac) transposase. Through homologous chromosome synapsis, recombination, and segregation, the Ds element separated from the Ac element. We selected stable Ds-only plants using G418 and GFP assays and analyzed 241 T-0 lines, some of which were highly efficient at producing Ds-only progeny. Through thermal asymmetric interlaced PCR, we isolated 710 independent Ds flanking sequences from Ds-only plants. Furthermore, we identified a large collection of mutants with visible developmental phenotypes via this transposon tagging system. The system is relatively simple and rapid in comparison to traditional T-DNA insertion strategies, because once efficiency lines are obtained they can be reused to generate more lines from nontransposed plants without the use of time-consuming tissue culture steps. |
| 学科主题 | Plant Sciences |
| 出版地 | CARY |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | AGROBACTERIUM-MEDIATED TRANSFORMATION ; FUNCTIONAL GENOMICS ; MODEL ; ELEMENTS ; LINES ; MUTAGENESIS ; SELECTION ; BIOLOGY ; TRAP ; AC |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000466860800013 |
| 出版者 | OXFORD UNIV PRESS INC |
| 资助机构 | Ministry of Agriculture and Rural Affairs of China [2016ZX08009-002] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2014CB138102] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570290] ; State Key Laboratory of Crop Biology [dxkt201705] ; Double Tops Project of Shandong Province |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19825]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Hongyu,Xue, Xiaodong,Qin, Caihua,et al. An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon[J]. PLANT PHYSIOLOGY,2019,180(1):56-65. |
| APA | Wu, Hongyu.,Xue, Xiaodong.,Qin, Caihua.,Xu, Yi.,Guo, Yuyu.,...&An, Hailong.(2019).An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon.PLANT PHYSIOLOGY,180(1),56-65. |
| MLA | Wu, Hongyu,et al."An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon".PLANT PHYSIOLOGY 180.1(2019):56-65. |
入库方式： OAI收割
来源：植物研究所
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