Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism
文献类型：期刊论文
| 作者 | Xue, Shan1; Zou, Junjie4; Liu, Yangfan1; Wang, Ming1,3; Zhang, Chunxia; Le, Jie
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2019 |
| 卷号 | 20期号:9 |
| 关键词 | ARF protein BIG family ARF-GEF auxin brassinosteroids gravity response |
| DOI | 10.3390/ijms20092339 |
| 文献子类 | Article |
| 英文摘要 | ADP-ribosylation factor-guanine nucleotide exchange factors (ARF-GEFs) act as key regulators of vesicle trafficking in all eukaryotes. In Arabidopsis, there are eight ARF-GEFs, including three members of the GBF1 subfamily and five members of the BIG subfamily. These ARF-GEFs have different subcellular localizations and regulate different trafficking pathways. Until now, the roles of these BIG-subfamily ARF-GEFs have not been fully revealed. Here, analysis of the BIGs expression patterns showed that BIG3 and BIG5 have similar expression patterns. big5-1 displayed a dwarf growth and big3-1 big5-1 double mutant showed more severe defects, indicating functional redundancy between BIG3 and BIG5. Moreover, both big5-1 and big3-1 big5-1 exhibited a reduced sensitivity to Brassinosteroid (BR) treatment. Brefeldin A (BFA)-induced BR receptor Brassinosteroid insensitive 1 (BRI1) aggregation was reduced in big5-1 mutant, indicating that the action of BIG5 is required for BRI1 recycling. Furthermore, BR-induced dephosphorylation of transcription factor BZR1 was decreased in big3-1 big5-1 double mutants. The introduction of the gain-of-function of BZR1 mutant BZR1-1D in big3-1 big5-1 mutants can partially rescue the big3-1 big5-1 growth defects. Our findings revealed that BIG5 functions redundantly with BIG3 in plant growth and gravitropism, and BIG5 participates in BR signal transduction pathway through regulating BRI1 trafficking. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 出版地 | BASEL |
| 电子版国际标准刊号 | 1422-0067 |
| WOS关键词 | BRASSINOSTEROID INSENSITIVE1 ; MEDIATES ENDOCYTOSIS ; AUXIN GRADIENTS ; GENE-EXPRESSION ; CELL-DIVISION ; ARABIDOPSIS ; RECEPTOR ; GOLGI ; KINASE ; GNOM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000469753500286 |
| 出版者 | MDPI |
| 资助机构 | National Basic Research Program of ChinaNational Basic Research Program of China [2011CB710902] ; Strategic Pioneer Program on Space Science, Chinese Academy of Sciences [XDA04079100] ; National Natural Science Foundation of China programNational Natural Science Foundation of China (NSFC) [30971652, 31271463] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19835]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, CAS Ctr Excellence Mol Plant Sci, Beijing 100093, Peoples R China 2.GeneFirst Ltd, Abingdon OX14 3DB, Oxon, England 3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Shan,Zou, Junjie,Liu, Yangfan,et al. Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(9). |
| APA | Xue, Shan,Zou, Junjie,Liu, Yangfan,Wang, Ming,Zhang, Chunxia,&Le, Jie.(2019).Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(9). |
| MLA | Xue, Shan,et al."Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.9(2019). |
入库方式： OAI收割
来源：植物研究所
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