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A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division

文献类型：期刊论文

作者Yang, Kezhen; Zhu, Lingling5; Wang, Hongzhe; Jiang, Min; Xiao, Chunwang2,4; Hu, Xiangyang6; Vanneste, Steffen7,8,9; Dong, Juan1; Le, Jie5
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2019
卷号116期号:36页码:18126-18131
关键词stomatal development cell division replication protein A CDK DNA damage
ISSN号0027-8424
DOI10.1073/pnas.1819345116
文献子类Article
英文摘要The R2R3-MYB transcription factor FOUR LIPS (FLP) controls the stomatal terminal division through transcriptional repression of the cell cycle genes CYCLIN-DEPENDENT KINASE (CDK) B1s (CDKB1s), CDKA; 1, and CYCLIN A2s (CYCA2s). We mutagenized the weak mutant allele flp-1 seeds with ethylmethane sulfonate and screened out a flp-1 suppressor 1 (fsp1) that suppressed the flp-1 stomatal cluster phenotype. FSP1 encodes RPA2a subunit of Replication Protein A (RPA) complexes that play important roles in DNA replication, recombination, and repair. Here, we show that FSP1/RPA2a functions together with CDKB1s and CYCA2s in restricting stomatal precursor proliferation, ensuring the stomatal terminal division and maintaining a normal guard-cell size and DNA content. Furthermore, we provide direct evidence for the existence of an evolutionarily conserved, but plant-specific, CDK-mediated RPA regulatory pathway. Serine-11 and Serine-21 at the N terminus of RPA2a are CDK phosphorylation target residues. The expression of the phosphorylation-mimic variant RPA2a(S11,21/D) partially complemented the defective cell division and DNA damage hypersensitivity in cdkb1;1 1;2 mutants. Thus, our study provides a mechanistic understanding of the CDK-mediated phosphorylation of RPA in the precise control of cell cycle and DNA repair in plants.
学科主题Multidisciplinary Sciences
出版地WASHINGTON
WOS关键词CYCLIN-DEPENDENT KINASE ; RPA PHOSPHORYLATION ; CELL-PROLIFERATION ; DAMAGE RESPONSE ; 4 LIPS ; SUBUNIT ; BINDING ; REPAIR ; CDC2 ; MUTATIONS
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000485140300076
出版者NATL ACAD SCIENCES
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31071198, 30971652, 31271463, 31470348] ; Research Foundation FlandersFWO ; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM109080] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM109080] Funding Source: NIH RePORTER
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/19841]  
专题中科院植物分子生理学重点实验室
作者单位1.Ghent Univ Global Campus, Lab Plant Growth Anal, Incheon 21985, South Korea
2.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
3.Rutgers State Univ, Waksman Inst Microbiol, Piscataway, NJ 08854 USA
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
6.Minzu Univ China, Hulun Lake Reserve Grassland Ecol Res Stn, Beijing 100081, Peoples R China
7.Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China
8.VIB, Ctr Plant Syst Biol, B-9052 Ghent, Belgium
9.Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium
推荐引用方式
GB/T 7714		 Yang, Kezhen,Zhu, Lingling,Wang, Hongzhe,et al. A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(36):18126-18131.
APA Yang, Kezhen.,Zhu, Lingling.,Wang, Hongzhe.,Jiang, Min.,Xiao, Chunwang.,...&Le, Jie.(2019).A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(36),18126-18131.
MLA Yang, Kezhen,et al."A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.36(2019):18126-18131.

入库方式： OAI收割

来源：植物研究所

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