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Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency

文献类型：期刊论文

作者Zhao, Taolan2,6; Huan, Qing2,6; Sun, Jing2; Liu, Chunyan2; Hou, Xiuli; Yu, Xiang; Silverman, Ian M.; Zhang, Yi1,9; Gregory, Brian D.; Liu, Chun-Ming
刊名GENOME BIOLOGY
出版日期2019
卷号20期号:1
关键词Poly(A) tails Poly(A)-binding proteins PAB binding efficiency Poly(A)-tail G-content mRNA stability Translational efficiency Arabidopsis
ISSN号1474-760X
DOI10.1186/s13059-019-1799-8
文献子类Article
英文摘要Background Polyadenylation plays a key role in producing mature mRNAs in eukaryotes. It is widely believed that the poly(A)-binding proteins (PABs) uniformly bind to poly(A)-tailed mRNAs, regulating their stability and translational efficiency. Results We observe that the homozygous triple mutant of broadly expressed Arabidopsis thaliana PABs, AtPAB2, AtPAB4, and AtPAB8, is embryonic lethal. To understand the molecular basis, we characterize the RNA-binding landscape of these PABs. The AtPAB-binding efficiency varies over one order of magnitude among genes. To identify the sequences accounting for the variation, we perform poly(A)-seq that directly sequences the full-length poly(A) tails. More than 10% of poly(A) tails contain at least one guanosine (G); among them, the G-content varies from 0.8 to 28%. These guanosines frequently divide poly(A) tails into interspersed A-tracts and therefore cause the variation in the AtPAB-binding efficiency among genes. Ribo-seq and genome-wide RNA stability assays show that AtPAB-binding efficiency of a gene is positively correlated with translational efficiency rather than mRNA stability. Consistently, genes with stronger AtPAB binding exhibit a greater reduction in translational efficiency when AtPAB is depleted. Conclusions Our study provides a new mechanism that translational efficiency of a gene can be regulated through the G-content-dependent PAB binding, paving the way for a better understanding of poly(A) tail-associated regulation of gene expression.
学科主题Biotechnology & Applied Microbiology ; Genetics & Heredity
出版地LONDON
WOS关键词POLY(A)-BINDING PROTEIN ; RNA-BINDING ; INITIATION-FACTOR ; REVEALS ; TAIL ; GENE ; MULTIPLE ; TRANSCRIPTS ; ALIGNMENT ; COMPLEX
WOS研究方向Biotechnology & Applied Microbiology ; Genetics & Heredity
语种英语
WOS记录号WOS:000483772800002
出版者BMC
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31788103, 91540203, 31770874, 31900455, 31571308, 31701096] ; National Basic Research Program of ChinaNational Basic Research Program of China [2014CB943400] ; Strategic Priority Research Program of Chinese Academy of SciencesChinese Academy of Sciences [XDPB0403, XDB27030201, XDA08020303] ; Key Research Program of Frontier Sciences of Chinese Academy of Sciences [QYZDY-SSW-SMC022] ; State Key Laboratory of Plant Genomics
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/19880]  
专题中科院植物分子生理学重点实验室
作者单位1.ABLife Inc, Lab Genome Regulat & Human Hlth, Wuhan 430075, Hubei, Peoples R China
2.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China
3.ABLife Inc, Ctr Genome Anal, Wuhan 430075, Hubei, Peoples R China
4.Chinese Acad Sci, Inst Genet & Dev Biol, CAS Ctr Excellence Mol Plant Sci, Beijing 100101, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China
7.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Genet Network Biol, Beijing 100101, Peoples R China
8.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
9.Yu, Xiang; Silverman, Ian M.; Gregory, Brian D.] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA
推荐引用方式
GB/T 7714		 Zhao, Taolan,Huan, Qing,Sun, Jing,et al. Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency[J]. GENOME BIOLOGY,2019,20(1).
APA Zhao, Taolan.,Huan, Qing.,Sun, Jing.,Liu, Chunyan.,Hou, Xiuli.,...&Cao, Xiaofeng.(2019).Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency.GENOME BIOLOGY,20(1).
MLA Zhao, Taolan,et al."Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency".GENOME BIOLOGY 20.1(2019).

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来源：植物研究所

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