Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa)
文献类型：期刊论文
| 作者 | Zheng, Guangshun1,5; Fan, Cunying1,5; Di, Shaokang; Wang, Xuemin; Gao, Liping4; Dzyubenko, Nikolay3; Chapurin, Vladimir3; Pang, Yongzhen |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2019 |
| 卷号 | 14期号:7 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0218336 |
| 文献子类 | Article |
| 英文摘要 | Flavonoids are one of the largest secondary metabolite groups, which are widely present in plants. Flavonoids include anthocyanins, proanthocyanidins, flavonols and isoflavones. In particular, proanthocyanidins possess beneficial effects for ruminant animals in preventing lethal pasture bloat. As a major legume forage, alfalfa (Medicago sativa) contains little proanthocyanidins in foliage to combat bloat. In an attempt to improve proanthocyanidin content in alfalfa foliage, we over-expressed two MYB transcription factors (CsMYB5-1 and CsMYB5-2) from tea plant that is rich in proanthocyanidins. We showed that, via targeted metabolite and transcript analyses, the transgenic alfalfa plants accumulated higher levels of flavonoids in stems/leaves than the control, in particular anthocyanins and proanthocyanidins. Over-expression of CsMYB5-1 and CsMYB5-2 induced the expression levels of genes involved in flavonoid pathway, especially anthocyanin/proanthocyanidin-specific pathway genes DFR, ANS and ANR in stems/leaves. Both anthocyanin/proanthocyanidin content and the expression levels of several genes were conversely decreased in flowers of the transgenic lines than in control. Our results indicated that CsMYB5-1 and CsMYB5-2 differently regulate anthocyanins/proanthocyanidins in stems/leaves and flowers. Our study provides a guide for increasing anthocyanin/proanthocyanidin accumulation in foliage of legume forage corps by genetic engineering. These results also suggest that it is feasible to cultivate new varieties for forage production to potentially solve pasture bloat, by introducing transcription factors from typical plants with high proanthocyanidin level. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | REGULATES PROANTHOCYANIDIN SYNTHESIS ; TRANSCRIPTION FACTOR ; SEED COAT ; BIOSYNTHESIS ; ANTHOCYANIN ; TRUNCATULA ; R2R3-MYB ; DIFFERENTIATION ; TRANSFORMATION ; GRAPEVINE |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000484921200009 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | Major State Basic Research and Development ProgramNational Basic Research Program of China [2014CB138703] ; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670305] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19886]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Russian Vavilov Inst Plant Genet Resources, Fed Res Ctr, St Petersburg, Russia 3.Anhui Agr Univ, Sch Life Sci, Hefei, Anhui, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China 5.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Guangshun,Fan, Cunying,Di, Shaokang,et al. Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa)[J]. PLOS ONE,2019,14(7). |
| APA | Zheng, Guangshun.,Fan, Cunying.,Di, Shaokang.,Wang, Xuemin.,Gao, Liping.,...&Pang, Yongzhen.(2019).Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa).PLOS ONE,14(7). |
| MLA | Zheng, Guangshun,et al."Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa)".PLOS ONE 14.7(2019). |
入库方式： OAI收割
来源：植物研究所
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