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Label-free SERS detection of Raman-Inactive protein biomarkers by Raman reporter indicator: Toward ultrasensitivity and universality

文献类型：期刊论文

作者Arabi, Maryam1; Ostovan, Abbas1; Zhang, Zhiyang1; Wang, Yunqing1,4; Mei, Rongchao1; Fu, Longwen1,4; Wang, Xiaoyan1; Chen, Lingxin1,4; Wang, Xiaoyan2; Ma, Jiping3
刊名BIOSENSORS & BIOELECTRONICS
出版日期2021-02-15
卷号174页码:112825
ISSN号0956-5663
关键词Surface-enhanced Raman scattering Raman reporter indicator mechanism Capillary sensor Mussel-inspired molecular imprinting Bioassay
DOI10.1016/j.bios.2020.112825
英文摘要It is still challenging to sensitively detect protein biomarkers via surface-enhanced Raman scattering (SERS) technique owing to their low Raman activity. SERS tag-based immunoassay is usually applied; however, it is laborious and needs specific antibodies. Herein, an ultrasensitive and universal Raman indicator sensing strategy is proposed for protein biomarkers, with the aid of a glass capillary-based molecularly imprinted SERS sensor. The sensor consists of an inner SERS substrate layer for signal enhancement and an outer mussel-inspired polydopamine imprinted layer as a recognition element. Imprinted cavities have two missions: first, selectively capturing the target protein, and second, the only passageway of Raman indicator to access SERS substrate. Specific protein recognition means filling imprinted cavities and blocking Raman indicator flow. Thus, the quantity of captured protein can be reflected by the signal decrease of ultra-Raman active indicator molecule. The capillary sensor exhibited specific and reproducible detection at the level down to 4.1 x 10(-3) mu g L-1, for trypsin enzyme in as-received biological samples without sample preparation. The generality of the mechanism is confirmed by using three different protein models. This platform provides a facile, fast and general route for sensitive SERS detection of Raman inactive biomacromolecules, which offers great promising utility for in situ and fast point-of-care practical bioassay.
WOS关键词IMPRINTED POLYMERS ; RECOGNITION ; SCATTERING ; GRAPHENE ; SENSORS ; ASSAY
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000605488400002
资助机构Chinese Academy of Sciences President's International Fellowship Initiative [2019PC0050, 2020PC0083] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [42076199, 21976209, 21804010, 22006162, 21976099, 81573393] ; Science and Technology Development Plan of Shandong Province of China [2019GSF108047] ; Youth Innovation Promotion Association CAS [2017256] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662] ; Taishan Scholar Project Special Funding [Ts20190962] ; Technical Innovation Project of Instrument Function Development of the Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/30514]  
专题烟台海岸带研究所_山东省海岸带环境工程技术研究中心
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China;
3.Qingdao Univ Technol, Sch Environm & Municipal Engn, Qingdao 266033, Peoples R China;
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Arabi, Maryam,Ostovan, Abbas,Zhang, Zhiyang,et al. Label-free SERS detection of Raman-Inactive protein biomarkers by Raman reporter indicator: Toward ultrasensitivity and universality[J]. BIOSENSORS & BIOELECTRONICS,2021,174:112825.
APA Arabi, Maryam.,Ostovan, Abbas.,Zhang, Zhiyang.,Wang, Yunqing.,Mei, Rongchao.,...&Zhang, Zhiyang.(2021).Label-free SERS detection of Raman-Inactive protein biomarkers by Raman reporter indicator: Toward ultrasensitivity and universality.BIOSENSORS & BIOELECTRONICS,174,112825.
MLA Arabi, Maryam,et al."Label-free SERS detection of Raman-Inactive protein biomarkers by Raman reporter indicator: Toward ultrasensitivity and universality".BIOSENSORS & BIOELECTRONICS 174(2021):112825.

入库方式： OAI收割

来源：烟台海岸带研究所

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