一株单环刺螠肠道电活性希瓦氏菌Shewanella marisflavI的生理学特性
文献类型:期刊论文
作者 | 李新1,2; 张月超1,2; 刘芳华1 |
刊名 | 微生物学报 |
出版日期 | 2020 |
卷号 | 60期号:7页码:1401-1412 |
ISSN号 | 0001-6209 |
关键词 | 肠道微生物 单环刺螠 异化铁、锰还原 电化学活性 |
其他题名 | Physiological characteristics of an intestinal electroactive strain Shewanella marisflavi UU-3-2 from Urechis unicinctus |
文献子类 | Article |
英文摘要 | [Objective] To explore the electroactive microorganism in the intestinal tract of marine annelids represented by Urechis unicinctus and characterizing its physiological and electrochemical properties.[Methods] The strain was isolated using plate scribing and identified by 16S rRNA gene sequencing.The morphology of the isolate was depicted by scanning electron microscope.High performance liquid chromatography(HPLC)was used to analyze the metabolites.The quantifications of Fe(Ⅱ)and Mn(Ⅱ)reduction were carried out by ferrozine and formaldoxime assays,respectively.Single-chamber microbial fuel cells(SCMFC)were used to test the electrochemical activity by cyclic voltammetry.[Results] A facultative anaerobic bacterium,Shewanella marisflavi UU-3-2(with the similarity of 99.93%),was successfully isolated which was a rod bacterium with the length about 2 mum and width about 0.5 mum.The metabolic analysis showed that this strain could use sodium lactate as an electron donor,fumarate as an electron acceptor for anaerobic respiration with acetate and succinate production.Ferrozine and formaldoxime assays illustrated that Fe(Ⅲ)/Mn(Ⅳ)in the Fe_2O_3/MnO_2 were reduced.The maximum current density was 146 mA/m~2,and the results of cyclic voltammetry revealed that the oxidation peak and reduction peak happened at 0.14 V and -0.51 V,respectively.[Conclusion] An electrochemical activity microbe,Shewanella marisflavi UU-3-2,was successfully isolated from marine animals represented by Urechis unicinctus,indicating the widespread existence of electrochemical activity microorganisms in marine annelids' intestine. |
语种 | 中文 |
CSCD记录号 | CSCD:6764318 |
资助机构 | 国家自然科学基金 ; 山东省自然科学杰出青年基金 ; 泰山学者青年专家计划 |
源URL | [http://ir.yic.ac.cn/handle/133337/30348] |
专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
作者单位 | 1.中国科学院烟台海岸带研究所,海岸带生物学与生物资源利用重点实验室,山东烟台264003; 2.中国科学院大学,北京100049 |
推荐引用方式 GB/T 7714 | 李新,张月超,刘芳华. 一株单环刺螠肠道电活性希瓦氏菌Shewanella marisflavI的生理学特性[J]. 微生物学报,2020,60(7):1401-1412. |
APA | 李新,张月超,&刘芳华.(2020).一株单环刺螠肠道电活性希瓦氏菌Shewanella marisflavI的生理学特性.微生物学报,60(7),1401-1412. |
MLA | 李新,et al."一株单环刺螠肠道电活性希瓦氏菌Shewanella marisflavI的生理学特性".微生物学报 60.7(2020):1401-1412. |
入库方式: OAI收割
来源:烟台海岸带研究所
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