LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States
文献类型：期刊论文
| 作者 | Liu, Wei1,2 ; Li, Yan1,2; Bai, Xue1; Wu, Haiguang1,3; Bian, Lanxing1,3; Hu, Xiaoke1,2; Bai, Xue3
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| 刊名 | MOLECULAR PLANT-MICROBE INTERACTIONS
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| 出版日期 | 2020-03 |
| 卷号 | 33期号:3页码:528-538 |
| 关键词 | Azorhizobium caulinodans HTH domain LuxR-type regulator REC domain Rhizobium-legume symbiosis |
| ISSN号 | 0894-0282 |
| DOI | 10.1094/MPMI-10-19-0306-R |
| 英文摘要 | LuxR-type regulators play important roles in transcriptional regulation in bacteria and control various biological processes. A genome sequence analysis showed the existence of seven LuxR-type regulators in Azorhizobium caulinodans ORS571, an important nitrogen-fixing bacterium in both its free-living state and in symbiosis with its host, Sesbania rostrata. However, the functional mechanisms of these regulators remain unclear. In this study, we identified a LuxR-type regulator that contains a cheY-homologous receiver (REC) domain in its N terminus and designated it AclR1. Interestingly, phylogenetic analysis revealed that AclR1 exhibited relatively close evolutionary relationships with MalT/GerE/FixJ/NarL family proteins. Functional analysis of an aclR1 deletion mutant (Delta aclR1) in the free-living state showed that AclR1 positively regulated cell motility and flocculation but negatively regulated exopoly-saccharide production, biofilm formation, and second messenger cyclic diguanylate (c-di-GMP)-related gene expression. In the symbiotic state, the Delta aclR1 mutant was defective in competitive colonization and nodulation on host plants. These results suggested that AclR1 could provide bacteria with the ability to compete effectively for symbiotic nodulation. Overall, our results show that the REC-LuxR-type regulator AclR1 regulates numerous phenotypes both in the free-living state and during host plant symbiosis. |
| WOS关键词 | PROTEIN ; SYSTEM ; NODULATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000551075100015 |
| 资助机构 | Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2016CB44] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31800099,41576165, 31600009] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/30465]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China; 3.Yantai Univ, Coll Life Sci, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Wei,Li, Yan,Bai, Xue,et al. LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2020,33(3):528-538. |
| APA | Liu, Wei.,Li, Yan.,Bai, Xue.,Wu, Haiguang.,Bian, Lanxing.,...&Bai, Xue.(2020).LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States.MOLECULAR PLANT-MICROBE INTERACTIONS,33(3),528-538. |
| MLA | Liu, Wei,et al."LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States".MOLECULAR PLANT-MICROBE INTERACTIONS 33.3(2020):528-538. |
入库方式： OAI收割
来源：烟台海岸带研究所
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