Comparison of bovine serum albumin glycation by ribose and fructose in vitro and in vivo
文献类型:期刊论文
作者 | Mou, Lixian1,2,3,4,6; Hu, Pingdong1; Cao, Xiao1,6; Chen, Yue2,3,4; Xu, Yong6; He, Tao6; Wei, Yan1; He, Rongqiao1,5,6 |
刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE |
出版日期 | 2022 |
卷号 | 1868期号:1页码:13 |
通讯作者邮箱 | yanwei@ibp.ac.cn (y. wei) ; herq@ibp.ac.cn (r. he) |
ISSN号 | 0925-4439 |
关键词 | Ribose Fructose Glycation Monomer Cytotoxic Type 2 diabetes mellitus |
DOI | 10.1016/j.bbadis.2021.166283 |
产权排序 | 2 |
文献子类 | 实证研究 |
英文摘要 | Advanced glycation end products (AGEs) play a critical pathogenic role in the development of diabetic complications. Recent studies have shown that diabetes is associated with not only abnormal glucose metabolism but also abnormal ribose and fructose metabolism, although glucose is present at the highest concentration in humans. The glycation ability and contribution of ribose and fructose to diabetic complications remain unclear. Here, the glycation ability of ribose, fructose and glucose under a mimic physiological condition, in which the concentration of ribose or fructose was one-fiftieth that of glucose, was compared. Bovine serum albumin (BSA) was used as the working protein in our experiments. Ribose generated more AGEs and was markedly more cytotoxic to SH-SY5Y cells than fructose. The first-order rate constant of ribose glycation was found to be significantly greater than that of fructose glycation. LC-MS/MS analysis revealed 41 ribose-glycated Lys residues and 12 fructose-glycated residues. Except for the shared Lys residues, ribose reacted selectively with 17 Lys, while no selective Lys was found in fructose-glycated BSA. Protein conformational changes suggested that ribose glycation may induce BSA into amyloid-like monomers compared with fructose glycation. The levels of serum ribose were correlated positively with glycated serum protein (GSP) and diabetic duration in type 2 diabetes mellitus (T2DM), respectively. These results indicate that ribose has a greater glycation ability than fructose, while ribose largely contributes to the production of AGEs and provides a new insight to understand in the occurrence and development of diabetes complications. |
收录类别 | SCI |
WOS关键词 | NONENZYMATIC GLYCATION ; END-PRODUCTS ; TISSUE PROTEINS ; BINDING ; GLUCOSE ; ANS ; IDENTIFICATION ; GLYCOXIDATION ; HYPERTENSION ; SURFACE |
资助项目 | National Natural Science Foundation of China[NSFC 31670805] ; National Natural Science Foundation of China[31270868] |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:000710122800006 |
资助机构 | National Natural Science Foundation of China |
源URL | [http://ir.psych.ac.cn/handle/311026/40883] |
专题 | 心理研究所_中国科学院心理健康重点实验室 |
通讯作者 | Wei, Yan; He, Rongqiao |
作者单位 | 1.Univ Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, 15 Datun Rd, Beijing 100101, Peoples R China 2.Academician Expert Workstn Sichuan Prov, Luzhou 646000, Sichuan, Peoples R China 3.Nucl Med & Mol Imaging Key Lab Sichuan Prov, Luzhou 646000, Sichuan, Peoples R China 4.Southwest Med Univ, Affiliated Hosp, Dept Nucl Med, 25 Taiping St, Luzhou 646000, Sichuan, Peoples R China 5.Univ Chinese Acad Sci, Inst Psychol, CAS Key Lab Mental Hlth, Beijing 100101, Peoples R China 6.Southwest Med Univ, Basic Coll Med, Luzhou 646000, Sichuan, Peoples R China |
推荐引用方式 GB/T 7714 | Mou, Lixian,Hu, Pingdong,Cao, Xiao,et al. Comparison of bovine serum albumin glycation by ribose and fructose in vitro and in vivo[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,2022,1868(1):13. |
APA | Mou, Lixian.,Hu, Pingdong.,Cao, Xiao.,Chen, Yue.,Xu, Yong.,...&He, Rongqiao.(2022).Comparison of bovine serum albumin glycation by ribose and fructose in vitro and in vivo.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,1868(1),13. |
MLA | Mou, Lixian,et al."Comparison of bovine serum albumin glycation by ribose and fructose in vitro and in vivo".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 1868.1(2022):13. |
入库方式: OAI收割
来源:心理研究所
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