A new anti-lipopolysaccharide factor isoform (PtALF4) from the swimming crab Portunus trituberculatus exhibited structural and functional diversity of ALFs
文献类型：期刊论文
| 作者 | Liu, Yuan1,2; Cui, Zhaoxia1; Li, Xihong1,2; Song, Chengwen1,2 ; Li, Qianqian1,2; Wang, Shuangyan1,2
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2012-05-01 |
| 卷号 | 32期号:5页码:724-731 |
| 关键词 | Portunus trituberculatus Anti-lipopolysaccharide factor Expression analysis Antimicrobial activity |
| ISSN号 | 1050-4648 |
| 通讯作者 | Cui, ZX (reprint author), Chinese Acad Sci, EMBL, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Anti-lipopolysaccharide factors (ALFs) are the potent antimicrobial peptides that can bind and neutralize lipopolysaccharide (LPS). In this study, a new isoform of the ALF homologs (PtALF4) was cloned from eyestalk cDNA library of swimming crab Portunus trituberculatus. PtALF4 shared lower amino acid similarity with other ALFs, yet it contained the conserved LPS-binding domain and was clearly member of the ALF family. The genomic sequence of PtALF4 consisted of three exons interrupted by two introns. Several tandem repeats were found in both introns. Unlike most ALFs expressed in hemocytes, PtALF4 transcript was predominantly detected in eyestalk. After challenge with Vibrio alginolyticus, the temporal expression level of PtALF4 transcript in hemocytes showed a clear time-dependent response expression pattern with two significant peaks. The recombinant proteins of PtALF1, PtALF3 and PtALF4 revealed different antimicrobial activities against bacteria or fungus. These results together suggest that PtALF isoforms might be potent immune effectors to provide multiple protective functions against invading bacteria or fungus in P. trituberculatus. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2012.01.021 |
| 语种 | 英语 |
| WOS记录号 | WOS:000302667200013 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12260]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yuan,Cui, Zhaoxia,Li, Xihong,et al. A new anti-lipopolysaccharide factor isoform (PtALF4) from the swimming crab Portunus trituberculatus exhibited structural and functional diversity of ALFs[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(5):724-731. |
| APA | Liu, Yuan,Cui, Zhaoxia,Li, Xihong,Song, Chengwen,Li, Qianqian,&Wang, Shuangyan.(2012).A new anti-lipopolysaccharide factor isoform (PtALF4) from the swimming crab Portunus trituberculatus exhibited structural and functional diversity of ALFs.FISH & SHELLFISH IMMUNOLOGY,32(5),724-731. |
| MLA | Liu, Yuan,et al."A new anti-lipopolysaccharide factor isoform (PtALF4) from the swimming crab Portunus trituberculatus exhibited structural and functional diversity of ALFs".FISH & SHELLFISH IMMUNOLOGY 32.5(2012):724-731. |
入库方式： OAI收割
来源：海洋研究所
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