A novel C1qDC protein acting as pattern recognition receptor in scallop Argopecten irradians
文献类型：期刊论文
| 作者 | Wang, Leilei1,2; Wang, Lingling1; Kong, Pengfei1,2; Yang, Jialong1,2; Zhang, Huan1; Wang, Mengqiang1 ; Zhou, Zhi1; Qiu, Limei1; Song, Linsheng1
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2012-08-01 |
| 卷号 | 33期号:2页码:427-435 |
| 关键词 | Argopecten irradians C1qDC protein Pattern recognition receptor PAMPs binding Microbial agglutinating activity |
| ISSN号 | 1050-4648 |
| 通讯作者 | Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | The C1q domain containing (C1qDC) proteins refer to a family of proteins containing the versatile charge pattern recognition globular C1q domain in the C-terminus, which could bind various ligands including PAMPs and trigger a serial of immune response. In this study, a novel C1qDC protein was identified from Argopecten irradians (designated as AiC1qDC-2). Its full-length cDNA was of 1062 bp with an open reading frame of 720 bp encoding a polypeptide of 240 amino acids containing a typical gC1 q domain. This gC1q domain possessed the typical 10-stranded beta-sandwich fold with a jelly-roll topology common to all C1q family members, and shared high homology with most of the other identified gC1q domains. The mRNA transcripts of AiC1qDC-2 were mainly detected in hepatopancreas, and also marginally detectable in mantle, gonad, adductor, gill and hemocytes. Its relative expression level in hemocytes was significantly up-regulated after challenges of fungi Pichia pastoris GS115 (P < 0.05), Gram-positive bacteria Micrococcus luteus (P < 0.05) and Gram-negative bacteria Vibrio anguillarum (P < 0.05). The recombinant protein of AiC1qDC-2 (rAiC1qDC-2) could bind various PAMPs, including LPS, PGN, polyI:C, mannan, beta-1,3-glucan as well as Yeast-glucan, and displayed agglutinating activity to fungi P. pastoris GS115, Gram-positive bacteria Bacillus subtilis and Gram-negative bacteria Escherichia coli TOP10F as well as V. anguillarum. All these results indicated that AiC1qDC-2 could function as a pattern recognition receptor to recognize various PAMPs on different pathogens in the innate immune responses of scallop, and provided new clues to understand the role of invertebrate C1qDC proteins in the ancient complement system. (c) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2012.05.032 |
| 语种 | 英语 |
| WOS记录号 | WOS:000306632800031 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12262]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Leilei,Wang, Lingling,Kong, Pengfei,et al. A novel C1qDC protein acting as pattern recognition receptor in scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(2):427-435. |
| APA | Wang, Leilei.,Wang, Lingling.,Kong, Pengfei.,Yang, Jialong.,Zhang, Huan.,...&Song, Linsheng.(2012).A novel C1qDC protein acting as pattern recognition receptor in scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,33(2),427-435. |
| MLA | Wang, Leilei,et al."A novel C1qDC protein acting as pattern recognition receptor in scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 33.2(2012):427-435. |
入库方式： OAI收割
来源：海洋研究所
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