A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection
文献类型：期刊论文
| 作者 | Sun, Y.1,2; Hu, Y-H.1; Liu, C-S.3; Sun, L.1 |
| 刊名 | LETTERS IN APPLIED MICROBIOLOGY
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 55期号:6页码:420-426 |
| 关键词 | cross-protection delivery DNA vaccine Edwardsiella tarda Streptococcus iniae |
| ISSN号 | 0266-8254 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Edwardsiella tarda and Streptococcus iniae are important fish pathogens. We have reported previously a live E. tarda vaccine based on the attenuated strain TX5RM and a S. iniae DNA vaccine based on the antigen Sia10. In this study, we examined the possibility of constructing a cross-genus vaccine by taking advantage of the residual infectivity of TX5RM and using it as a carrier host for the natural delivery of a S. iniae DNA vaccine. For this purpose, the recombinant TX5RM, TX5RMS10, was created, which harbours and retains stably the DNA vaccine plasmid pCS10 that expresses Sia10. When flounder were vaccinated with TX5RMS10 via oral and immersion routes, TX5RMS10 was detected in multiple tissues within 12-14 days postvaccination (p.v.). At 7 and 14 days p.v., expression of the DNA vaccine was detected in spleen, kidney and liver. Following E. tarda and S. iniae challenge at one and 2 months p.v., the vaccinated fish exhibited relative per cent survival rates of 69-83%. Immunological analysis indicated that TX5RMS10-vaccinated fish produced specific serum antibodies and exhibited enhanced expression of a wide range of immune genes. |
| 学科主题 | Biotechnology & Applied Microbiology ; Microbiology |
| 收录类别 | SCI |
| 原文出处 | 10.1111/j.1472-765X.2012.03307.x |
| 语种 | 英语 |
| WOS记录号 | WOS:000314225000004 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12271]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Y.,Hu, Y-H.,Liu, C-S.,et al. A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection[J]. LETTERS IN APPLIED MICROBIOLOGY,2012,55(6):420-426. |
| APA | Sun, Y.,Hu, Y-H.,Liu, C-S.,&Sun, L..(2012).A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection.LETTERS IN APPLIED MICROBIOLOGY,55(6),420-426. |
| MLA | Sun, Y.,et al."A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection".LETTERS IN APPLIED MICROBIOLOGY 55.6(2012):420-426. |
入库方式： OAI收割
来源：海洋研究所
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