A trehalose-6-phosphate synthase gene from Chinese shrimp, Fenneropenaeus chinensis
文献类型：期刊论文
| 作者 | Zhang, Jiquan1; Wang, Jing1,2; Li, Fuhua1; Sun, Yuying1; Yang, Changjian1; Xiang, Jianhai1
|
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 39期号:12页码:10219-10225 |
| 关键词 | Fenneropenaeus chinensis Trehalose-6-phosphate synthase WSSV Vibrio |
| ISSN号 | 0301-4851 |
| 通讯作者 | Zhang, JQ (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 英文摘要 | Trehalose is an important disaccharide and plays a key role in many organisms under different stress conditions. In the study, a gene (FcTPS) encoded trehalose-6-phosphate synthase was reported from Chinese shrimp, Fenneropenaeus chinensis. The full-length cDNA of FcTPS is 3,281 bp including a poly A-tail of 20 bp, encoding a putative protein of 844 amino acids. The predicted protein contains a glycol_transf_20 domain and a trehalose_PPase domain. Genomic structure of FcTPS is composed of three exons with 192, 157 and 2,912 bp and two introns with 1,057 and 568 bp. In the second intron, four different SSRs are found. Transcripts of FcTPS gene are constitutively expressed in various tissues, with strongest level in hepatopancreas. After the shrimp were challenged with WSSV or Vibrio and the expression of FcTPS in hepatopancreas were analyzed using real-time PCR, the result showed that FcTPS transcript was down-regulated significantly in response to the challenge of Vibro at the early of 5 h post-challenge and then up-regulated significantly at 14 h. In addition, the expression of FcTPS showed the same result after the shrimp were challenged with WSSV. These results provide some new information about the tissue distribution, expression profiles and potential function of the trehalose-6-phosphate synthase in shrimp. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s11033-012-1897-0 |
| 语种 | 英语 |
| WOS记录号 | WOS:000310586700027 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12274]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jiquan,Wang, Jing,Li, Fuhua,et al. A trehalose-6-phosphate synthase gene from Chinese shrimp, Fenneropenaeus chinensis[J]. MOLECULAR BIOLOGY REPORTS,2012,39(12):10219-10225. |
| APA | Zhang, Jiquan,Wang, Jing,Li, Fuhua,Sun, Yuying,Yang, Changjian,&Xiang, Jianhai.(2012).A trehalose-6-phosphate synthase gene from Chinese shrimp, Fenneropenaeus chinensis.MOLECULAR BIOLOGY REPORTS,39(12),10219-10225. |
| MLA | Zhang, Jiquan,et al."A trehalose-6-phosphate synthase gene from Chinese shrimp, Fenneropenaeus chinensis".MOLECULAR BIOLOGY REPORTS 39.12(2012):10219-10225. |
入库方式： OAI收割
来源：海洋研究所
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