C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine
文献类型：期刊论文
| 作者 | Yu, Lan-Ping1,2; Hu, Yong-Hua1; Sun, Bo-Guang1 ; Sun, Li1
|
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2012-12-03 |
| 卷号 | 102期号:1页码:33-42 |
| 关键词 | Vibrio anguillarum Attenuated vaccine Rifampicin resistance Immersion Oral feeding |
| ISSN号 | 0177-5103 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Vibrio anguillarum, a Gram-negative bacterial pathogen, is the causative agent of vibriosis that affects a wide range of aquatic animals. In this study, we obtained a mutant V. anguillarum, C312M, derived from the pathogenic V. anguillarum C312 by selection of rifampicin resistance. C312M was slower in growth than the wild type C312, particularly under conditions of iron depletion. Compared to C312, C312M was altered in protein production profile and exhibited a dramatically increased median lethal dose. Safety analysis showed that C312M was stable in virulence in the absence of selective pressure. To examine the potential of C312M as a live attenuated vaccine, Japanese flounder Paralichthys olivaceus were vaccinated with C312M via oral, immersion, and oral plus immersion routes. Microbiological analysis showed that C312M was recovered from the gut, liver, kidney, and spleen of the vaccinated fish in 1 to 14 d post-vaccination. When the fish were challenged with C312 at 1 mo post-vaccination, C312M-vaccinated fish exhibited relative percent survival rates of 60 to 84%. Comparable protection was observed when the fish were challenged with a heterologous V. anguillarum strain. Further analysis showed that C312M-vaccinated fish produced specific serum antibodies which enhanced serum bactericidal activity in a manner that is probably complement-dependent. These results indicate that C312M is highly attenuated in virulence but still retains residual infectivity, and that C312M is an effective vaccine when delivered alive via immersion and oral feeding. |
| 学科主题 | Fisheries ; Veterinary Sciences |
| 收录类别 | SCI |
| 原文出处 | 10.3354/dao02544 |
| 语种 | 英语 |
| WOS记录号 | WOS:000311967600004 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12278]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Lan-Ping,Hu, Yong-Hua,Sun, Bo-Guang,et al. C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine[J]. DISEASES OF AQUATIC ORGANISMS,2012,102(1):33-42. |
| APA | Yu, Lan-Ping,Hu, Yong-Hua,Sun, Bo-Guang,&Sun, Li.(2012).C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine.DISEASES OF AQUATIC ORGANISMS,102(1),33-42. |
| MLA | Yu, Lan-Ping,et al."C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine".DISEASES OF AQUATIC ORGANISMS 102.1(2012):33-42. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。