Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis
文献类型：期刊论文
| 作者 | Guan XiangYu2; Zhang WeiJie3; Chi XiaoYuan3; Lin HanZhi3,4; Wang JinFeng3 ; Qin Song1
|
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2012-09-01 |
| 卷号 | 57期号:25页码:3294-3299 |
| 关键词 | Escherichia coli phycocyanin fluorescence recombinant |
| ISSN号 | 1001-6538 |
| 通讯作者 | Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. |
| 英文摘要 | To biosynthesize fluorescent Spirulina platensis (Sp) beta-phycocyanin (PC) in Escherichia coli, a BLASTP search for homologs of the cpeS gene, a chromophore lyase, was performed against the Synechocystis sp. PCC 6803 (S6) proteome. A highly homologous gene, slr2049, was obtained from the S6 genome. Sites 82 and 153 in beta-phycocyanin of Sp were modified by site-directed mutagenesis. Two recombinant expression vectors were constructed and transformed into E. coli BL21: (i) pCDF-cpcB (C153A)-slr2049-sll0583-ho1-pcyA; and (ii) pCDF-cpcB (C82I)-slr2049-sll0583-ho1-pcyA. Lyases encoded by the genes slr2049 and sll0583 catalyzed the linking of Sp 82 beta-PC to phycocyanobilin (PCB), and fluorescent CpcB (C153A)-PCB was generated. We present a strategy for the co-expression of multiple genes in a single expression vector to identify the function of an unknown gene. Recombinant phycobiliproteins produced on a large scale are promising fluorescent tags for diagnostics and pharmacology. |
| 学科主题 | Multidisciplinary Sciences |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s11434-012-5264-2 |
| 语种 | 英语 |
| WOS记录号 | WOS:000307750500007 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12302]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.China Univ Geosci, Sch Ocean Sci, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guan XiangYu,Zhang WeiJie,Chi XiaoYuan,et al. Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3294-3299. |
| APA | Guan XiangYu,Zhang WeiJie,Chi XiaoYuan,Lin HanZhi,Wang JinFeng,&Qin Song.(2012).Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis.CHINESE SCIENCE BULLETIN,57(25),3294-3299. |
| MLA | Guan XiangYu,et al."Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis".CHINESE SCIENCE BULLETIN 57.25(2012):3294-3299. |
入库方式： OAI收割
来源：海洋研究所
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