Construction and analysis of experimental DNA vaccines against megalocytivirus
文献类型：期刊论文
| 作者 | Zhang, Min; Hu, Yong-Hua; Xiao, Zhi-Zhong; Sun, Yun; Sun, Li |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2012-11-01 |
| 卷号 | 33期号:5页码:1192-1198 |
| 关键词 | Megalocytivirus DNA vaccine Scophthalmus maximus Immune response |
| ISSN号 | 1050-4648 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Iridoviruses are large double-stranded DNA viruses with icosahedral capsid. The Iridoviridae family contains five genera, one of which is Megalocytivirus. Megalocytivirus has emerged in recent years as an important pathogen to a wide range of marine and freshwater fish. In this study, we aimed at developing effective genetic vaccines against megalocytivirus affecting farmed fish in China. For this purpose, we constructed seven DNA vaccines based on seven genes of rock bream iridovirus isolate 1 from China (RBIV-C1), a megalocytivirus with a host range that includes Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus). The protective potentials of these vaccines were examined in a turbot model. The results showed that after vaccination via intramuscular injection, the vaccine plasmids were distributed in spleen, kidney, muscle, and liver, and transcription of the vaccine genes and production of the vaccine proteins were detected in these tissues. Following challenge with a lethal-dose of RBIV-C1, fish vaccinated with four of the seven DNA vaccines exhibited significantly higher levels of survival compared to control fish. Of these four protective DNA vaccines, pCN86, which is a plasmid that expresses an 86-residue viral protein, induced the highest protection. Immunological analysis showed that pCN86 was able to (i) stimulate the respiratory burst of head kidney macrophages at 14 d, 21 d, and 28 d post-vaccination, (ii) upregulate the expression of immune relevant genes involved in innate and adaptive immunity, and (iii) induce production of serum antibodies that, when incubated with RBIV-C1 before infection, significantly reduced viral loads in kidney and spleen following viral infection of turbot. Taken together, these results indicate that pCN86 is an effective DNA vaccine that may be used in the control of megalocytivirus-associated diseases in aquaculture. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2012.09.010 |
| 语种 | 英语 |
| WOS记录号 | WOS:000311191700015 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12305]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Hu, Yong-Hua,Xiao, Zhi-Zhong,et al. Construction and analysis of experimental DNA vaccines against megalocytivirus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(5):1192-1198. |
| APA | Zhang, Min,Hu, Yong-Hua,Xiao, Zhi-Zhong,Sun, Yun,&Sun, Li.(2012).Construction and analysis of experimental DNA vaccines against megalocytivirus.FISH & SHELLFISH IMMUNOLOGY,33(5),1192-1198. |
| MLA | Zhang, Min,et al."Construction and analysis of experimental DNA vaccines against megalocytivirus".FISH & SHELLFISH IMMUNOLOGY 33.5(2012):1192-1198. |
入库方式： OAI收割
来源：海洋研究所
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