Crustins from eyestalk cDNA library of swimming crab Portunus trituberculatus: Molecular characterization, genomic organization and expression analysis
文献类型：期刊论文
| 作者 | Cui, Zhaoxia1; Song, Chengwen1,2 ; Liu, Yuan1; Wang, Shuangyan1,2; Li, Qianqian1,2; Li, Xihong1,2
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2012-10-01 |
| 卷号 | 33期号:4页码:937-945 |
| 关键词 | Portunus trituberculatus Crustin Genomic organization Expression profile Antibacterial activity |
| ISSN号 | 1050-4648 |
| 通讯作者 | Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Crustins are cationic, cysteine-rich antimicrobial proteins, containing a single whey acidic protein (WAP) domain in the C-terminal end. Different from the reported Ptcrustin in the hemocytes, two novel crustin genes (PtCrustin2 and PtCrustin3) were cloned completely from the eyestalk cDNA library of Portunus trituberculatus in this study. All PtCrustins share the consensus cysteine motif and are considered as Type I crustins. Four exons and three introns are identified in genomic DNA sequence of PtCrustin3 while three exons and two introns in PtCrustin2. The mRNA transcripts of PtCrustin2 and PtCrustin3 are mainly detected in eyestalk and gills, but not in hemocytes. Although both PtCrutins are up-regulated after challenge of three microorganisms, PtCrustin3 seems to respond more quickly to microbial challenge than Ptcrustin2. Unlike most crustins, both recombinant PtCrustin2 and PtCrustin3 exhibit antibacterial activity against Gram-positive bacteria Micrococcus luteus and Staphyloccocus aureus and Gram-negative bacterium Pseudomonas aeruginosa. In addition, rPtCrustin2 is moderately active against yeast Pichia pastoris and rPtCrustin3 show significant activity against Gram-negative bacterium Vibrio alginolyticus. These results indicate that PtCrustin2 and PtCrustin3 are two novel crustins and play different roles in immune response of P. trituberculatus against microbial challenge. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2012.08.002 |
| 语种 | 英语 |
| WOS记录号 | WOS:000310185800030 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12308]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Zhaoxia,Song, Chengwen,Liu, Yuan,et al. Crustins from eyestalk cDNA library of swimming crab Portunus trituberculatus: Molecular characterization, genomic organization and expression analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(4):937-945. |
| APA | Cui, Zhaoxia,Song, Chengwen,Liu, Yuan,Wang, Shuangyan,Li, Qianqian,&Li, Xihong.(2012).Crustins from eyestalk cDNA library of swimming crab Portunus trituberculatus: Molecular characterization, genomic organization and expression analysis.FISH & SHELLFISH IMMUNOLOGY,33(4),937-945. |
| MLA | Cui, Zhaoxia,et al."Crustins from eyestalk cDNA library of swimming crab Portunus trituberculatus: Molecular characterization, genomic organization and expression analysis".FISH & SHELLFISH IMMUNOLOGY 33.4(2012):937-945. |
入库方式： OAI收割
来源：海洋研究所
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