Cynoglossus semilaevis thioredoxin: a reductase and an antioxidant with immunostimulatory property
文献类型：期刊论文
| 作者 | Sun, Jin-sheng2; Li, Yong-xin1,2; Sun, Li1 |
| 刊名 | CELL STRESS & CHAPERONES
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 17期号:4页码:445-455 |
| 关键词 | Thioredoxin Cynoglossus semilaevis Redox Antioxidant Oxidative stress Immunoregulatory |
| ISSN号 | 1355-8145 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Thioredoxin (Trx) is a small redox protein existing ubiquitously in all living organisms and plays an important role in multiple cellular processes, including transcriptional regulation and immune response. To date very few studies have been carried out to examine the function of piscine Trx. In this study, we identified and analyzed the function of a Trx homologue, CsTrx1, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsTrx1 is composed of 107 residues and shares 54.1-60.8% overall identities with the Trx of other teleosts. CsTrx1 contains the highly conserved CXXC motif, which in mammals is known to be the active site, in the form of CQPC. Expression of CsTrx1 as determined by quantitative real-time reverse transcriptase PCR was highest in liver and upregulated in time-dependent manners by bacterial infection and by exposure to iron, copper, and hydrogen peroxide. Purified recombinant CsTrx1 (rCsTrx1) exhibited insulin disulfide reductase activity and antioxidant activity, both which, however, were lost when the two cysteine residues in the CQPC motif were mutated to serine. Further analysis showed that rCsTrx1 was able to stimulate the proliferation of head kidney leukocytes, upregulate the expression of immune relevant genes, and enhance the resistance of leukocytes against bacterial infection. Taken together, these results indicate that CsTrx1 is a biologically active reductase and an antioxidant that requires the CXXC motif for activity and that CsTrx1 possesses cytokine-like immunoregulatory property. These results suggest a role for CsTrx1 in protecting cells against oxidative stress caused by oxidant exposure and pathogen infection. |
| 学科主题 | Cell Biology |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s12192-012-0322-x |
| 语种 | 英语 |
| WOS记录号 | WOS:000304927700004 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12310]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Cytogenet & Mol Regulat, Tianjin 300387, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Jin-sheng,Li, Yong-xin,Sun, Li. Cynoglossus semilaevis thioredoxin: a reductase and an antioxidant with immunostimulatory property[J]. CELL STRESS & CHAPERONES,2012,17(4):445-455. |
| APA | Sun, Jin-sheng,Li, Yong-xin,&Sun, Li.(2012).Cynoglossus semilaevis thioredoxin: a reductase and an antioxidant with immunostimulatory property.CELL STRESS & CHAPERONES,17(4),445-455. |
| MLA | Sun, Jin-sheng,et al."Cynoglossus semilaevis thioredoxin: a reductase and an antioxidant with immunostimulatory property".CELL STRESS & CHAPERONES 17.4(2012):445-455. |
入库方式： OAI收割
来源：海洋研究所
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