Edwardsiella ictaluri LuxS: Activity, Expression, and Involvement in Pathogenicity
文献类型：期刊论文
| 作者 | Zhang, Min; Sun, Li |
| 刊名 | POLISH JOURNAL OF MICROBIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 61期号:4页码:263-271 |
| 关键词 | Edwardsiella ictaluri AI-2 activity RNA interference quorum sensing virulence |
| ISSN号 | 1733-1331 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Edwardsiella ictaluri is a Gram-negative bacterium and the causative agent of enteric septicemia of catfish. In this study, we examined the expression and function of the LuxS from a pathogenic E. ictaluri strain, J901. J901 was found to produce autoinducer 2 (AI-2) activity that maximized at mid-logarithmic phase and was enhanced by glucose and repressed by high temperature. Consistently, a luxS gene (luxS(E1)) was identified in J901, whose expression was regulated by cell density, glucose, and temperature in a manner similar to that observed with AI-2 activity. Further analysis showed that LuxS(E1) is a biologically active AI-2 synthase that was able to complement the luxS-defective phenotype of Escherichia coli DH5 alpha. To examine the functional importance of LuxS(Ei), a genetically modified variant of J901, J901Ri, was constructed, in which luxS(E1) expression was blocked by RNA interference. Compared to the wild type, J901Ri was (i) reduced in AI-2 activity to a level of 59% of that of the wild type; (ii) impaired in both planktonic and biofilm growth; (iii) significantly attenuated in the ability to infect cultured fish cells and to cause mortality in infected fish; (iv) unable to induce the expression of certain virulence-associated genes. Addition of exogenous AI-2 failed to rescue the growth defect of J901Ri as free-living cells but restored biofilm production and the expression of virulence genes to levels comparable to those of the wild type. Taken together, these results indicate that LuxS(E1) is a functional AI-2 synthase that is required for optimal cellular growth and host infection. |
| 学科主题 | Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317088300004 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12313]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Sun, Li. Edwardsiella ictaluri LuxS: Activity, Expression, and Involvement in Pathogenicity[J]. POLISH JOURNAL OF MICROBIOLOGY,2012,61(4):263-271. |
| APA | Zhang, Min,&Sun, Li.(2012).Edwardsiella ictaluri LuxS: Activity, Expression, and Involvement in Pathogenicity.POLISH JOURNAL OF MICROBIOLOGY,61(4),263-271. |
| MLA | Zhang, Min,et al."Edwardsiella ictaluri LuxS: Activity, Expression, and Involvement in Pathogenicity".POLISH JOURNAL OF MICROBIOLOGY 61.4(2012):263-271. |
入库方式： OAI收割
来源：海洋研究所
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