EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica
文献类型：期刊论文
| 作者 | Liu, Fuli1,2; Wang, Feijiu1; Duan, Delin2 |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2012-06-01 |
| 卷号 | 24期号:3页码:501-505 |
| ISSN号 | 0921-8971 |
| 关键词 | Saccharina japonica Laminaria digitata EST SSR Phaeophyta |
| 通讯作者 | Wang, FJ (reprint author), Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China. |
| 英文摘要 | The public availability of numerous expressed sequence tag (EST) enables EST-based SSR (simple sequence repeat) markers to be widely used for genetics and breeding studies. In the present study, EST-SSR markers were developed from ESTs of and were transferred to the non-congeneric species . Among the 2,668 non-redundant ESTs, 83 (3.1%) ESTs containing SSR were identified totally, with an average of one SSR per 13.6 kb. Analysis of SSR motifs revealed that the trinucleotide and tetranucleotide were major motifs, accounted for 44.58% and 16.87%, respectively. Based on the 83 ESTs containing SSR, we designed 45 pairs of primers in the flanking regions of the SSR, of which 13 pairs showed polymorphism in a wild population, and the mean alleles per locus was 3.6 (ranging from 2 to 6). The observed (Ho) and expected (He) heterozygosities of these EST-SSRs were 0.234-0.632 and 0.260-0.635, respectively. All loci were in Hardy-Weinberg equilibrium in the wild population and no linkage disequilibrium was detected among loci. The obtained EST-SSR markers can facilitate and promote related research such as ecological investigation, genetic diversity assessment and breeding practice of as well. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s10811-012-9807-3 |
| 语种 | 英语 |
| WOS记录号 | WOS:000304187100024 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12324]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Fuli,Wang, Feijiu,Duan, Delin. EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica[J]. JOURNAL OF APPLIED PHYCOLOGY,2012,24(3):501-505. |
| APA | Liu, Fuli,Wang, Feijiu,&Duan, Delin.(2012).EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica.JOURNAL OF APPLIED PHYCOLOGY,24(3),501-505. |
| MLA | Liu, Fuli,et al."EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica".JOURNAL OF APPLIED PHYCOLOGY 24.3(2012):501-505. |
入库方式： OAI收割
来源：海洋研究所
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