Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5
文献类型：期刊论文
| 作者 | Wei, Maosheng1,2,3,4; Geng, Lihua1,2,3; Wang, Qingchi1,2,3,4 ; Yue, Yang1,2,3; Wang, Jing1,2,3; Wu, Ning1,2,3; Wang, Xiaoqing1,2,3,4; Sun, Chaomin1,2,3; Zhang, Quanbin1,2,3
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 出版日期 | 2021-10-31 |
| 卷号 | 189页码:649-656 |
| 关键词 | Exopolysaccharide Characteristics Immunostimulatory effects |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2021.08.159 |
| 通讯作者 | Geng, Lihua(lhgeng@qdio.ac.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn) |
| 英文摘要 | Crude exopolysaccharides from extracellular polymeric substances produced by the marine bacterium Bacillus sp. H5 were fractionated using DEAE-Sepharose FF and Sephadex G-75 chromatography. The high molecular weight fraction (89.0 kD) from the neutral fraction was designated EPS5SH; it contained mannose, glucosamine, glucose, and galactose in a molar ratio of 1.00: 0.02: 0.07: 0.02. Infra-red, gas chromatography-mass spectrometry, electrospray ionisation-tandem mass spectrometry analysis and nuclear magnetic resonance revealed EPS5SH was a mannan with alpha-(1-* 4)-Manp, alpha-(1-* 2)-Manp, alpha-(1-* 4, 6)-Manp and 8-terminal-Manp. Preliminary in vitro experiments revealed that EPS5SH significantly upregulated nitric oxide synthesis and release of pro inflammatory factors in murine macrophage RAW264.7 cells. Western blot experiments verified the immunostimulatory effects of EPS5SH through the modulation of the NF-kappa B and MAPK signalling pathways. In conclusion, EPS5SH was a novel immunostimulatory mannan. |
| 资助项目 | China Ocean Mineral Resources RD Association[DY135-B2-14] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000702873200006 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/176529]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Geng, Lihua; Zhang, Quanbin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS, Ctr Ocean Megasci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Megasci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Maosheng,Geng, Lihua,Wang, Qingchi,et al. Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,189:649-656. |
| APA | Wei, Maosheng.,Geng, Lihua.,Wang, Qingchi.,Yue, Yang.,Wang, Jing.,...&Zhang, Quanbin.(2021).Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,189,649-656. |
| MLA | Wei, Maosheng,et al."Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 189(2021):649-656. |
入库方式： OAI收割
来源：海洋研究所
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