Identification and characterization of a serine protease inhibitor (PtSerpin) in the swimming crab Portunus trituberculatus
文献类型：期刊论文
| 作者 | Wang, Shuangyan1,2; Cui, Zhaoxia1; Liu, Yuan1; Li, Qianqian1,2 ; Song, Chengwen1,2
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 32期号:4页码:544-550 |
| 关键词 | Portunus trituberculatus Prophenoloxidase Serpin Expression profile Immunity |
| ISSN号 | 1050-4648 |
| 通讯作者 | Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Serine protease inhibitors (Serpins) play a key role in diverse immune biological processes. A serine protease inhibitor (Serpin), namely PtSerpin, was identified from the haemocyte cDNA library of swimming crab Portunus trituberculatus. The full-length PtSerpin cDNA was 1593 bp, including an open reading frame (ORF) of 1227 bp encoding a polypeptide of 408 amino acids with estimated molecular mass of 45.048 kDa and theoretical isoelectric point of 7.23. Predicted tertiary structure of PtSerpin contained three beta-sheets and nine alpha-helices. Multiple sequence alignment revealed that deduced amino acid sequence of PtSerpin shared the highest similarity with serpin SPI from green mud crab Scylla paramamosain (SpSerpin). Phylogenetic analysis supported PtSerpin and SpSerpin were closely related to serpins from Penaeus monodon and Daphnia pulex while other decapods formed a separate group. Although the mRNA transcripts of PtSerpin could be detected in all the examined tissues, the higher levels were present in haemocytes and gills which are the major organs respond to pathogenic microorganism. After challenged by Vibrio alginolyticus, Micrococcus luteus and Pichia pas forts, the temporal expression of PtSerpin gene in haemocytes showed different activation times against bacteria and fungi within the experimental period of 72 h. These findings suggest that PtSerpin is involved in the antibacterial defense mechanism of P. trituberculatus crab. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2012.01.002 |
| 语种 | 英语 |
| WOS记录号 | WOS:000301827100005 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12344]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shuangyan,Cui, Zhaoxia,Liu, Yuan,et al. Identification and characterization of a serine protease inhibitor (PtSerpin) in the swimming crab Portunus trituberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(4):544-550. |
| APA | Wang, Shuangyan,Cui, Zhaoxia,Liu, Yuan,Li, Qianqian,&Song, Chengwen.(2012).Identification and characterization of a serine protease inhibitor (PtSerpin) in the swimming crab Portunus trituberculatus.FISH & SHELLFISH IMMUNOLOGY,32(4),544-550. |
| MLA | Wang, Shuangyan,et al."Identification and characterization of a serine protease inhibitor (PtSerpin) in the swimming crab Portunus trituberculatus".FISH & SHELLFISH IMMUNOLOGY 32.4(2012):544-550. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。