Identification and Characterization of the Sex-Determiner Transformer-2 Homologue in Chinese Shrimp, Fenneropenaeus chinensis
文献类型：期刊论文
| 作者 | Li, S.1 ; Li, F.1; Wen, R.1,2; Xiang, J.1
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| 刊名 | SEXUAL DEVELOPMENT
 |
| 出版日期 | 2012 |
| 卷号 | 6期号:5页码:267-278 |
| 关键词 | Fenneropenaeus chinensis Larval stage Sex determination Transformer-2 gene |
| ISSN号 | 1661-5425 |
| 通讯作者 | Li, F (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | The transformer-2 gene, encoding a protein (Tra-2) which directs sex-specifically alternative splicing of doublesex (dsx) pre-mRNA in combination with the transformer (Tra) protein, has been proved to play important roles on sex differentiation and sex development in Drosophila melanogaster. In the present study, a tra-2 homologue (FcTra-2) was cloned and characterized in the Chinese shrimp, Fenneropenaeus chinensis. A FcTra-2 genomic DNA sequence with a length of 8,871 bp was obtained and verified to consist of 7 exons and 6 introns. Three alternatively spliced mRNA transcripts, designated as FcTra-2a, FcTra-2b and FcTra-2c, were isolated and characterized. Sequence analysis showed that FcTra-2 included a RNA recognition motif and a linker region, which shared high sequence identities with Tra-2 from other species and 2 arginine/serine rich regions. Further studies were performed on the isoform FcTra-2c, since it exhibited a significantly higher expression level in ovary than in other tissues. In early developmental stages of the shrimp, FcTra-2c was detected to suddenly increase its expression level at the mysis stage. In juvenile stage, FcTra-2c displayed a significantly higher expression level in female Chinese shrimp than in males. These data indicated that FcTra-2 might be involved in female sex determination in Chinese shrimp. Copyright (C) 2012 S. Karger AG, Basel |
| 学科主题 | Developmental Biology |
| 收录类别 | SCI |
| 原文出处 | 10.1159/000341377 |
| 语种 | 英语 |
| WOS记录号 | WOS:000307608100006 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12345]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, S.,Li, F.,Wen, R.,et al. Identification and Characterization of the Sex-Determiner Transformer-2 Homologue in Chinese Shrimp, Fenneropenaeus chinensis[J]. SEXUAL DEVELOPMENT,2012,6(5):267-278. |
| APA | Li, S.,Li, F.,Wen, R.,&Xiang, J..(2012).Identification and Characterization of the Sex-Determiner Transformer-2 Homologue in Chinese Shrimp, Fenneropenaeus chinensis.SEXUAL DEVELOPMENT,6(5),267-278. |
| MLA | Li, S.,et al."Identification and Characterization of the Sex-Determiner Transformer-2 Homologue in Chinese Shrimp, Fenneropenaeus chinensis".SEXUAL DEVELOPMENT 6.5(2012):267-278. |
入库方式： OAI收割
来源：海洋研究所
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