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The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles

文献类型：期刊论文

作者Xu, Chaojie1,2,3,4; Xing, Ronge1,2,3; Liu, Song1,2,3; Qin, Yukun1,2,3; Li, Kecheng1,2,3; Yu, Huahua1,2,3; Li, Pengcheng1,2,3
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2021-06-30
卷号181页码:398-409
关键词Carboxymethyl chitosan DCS cells Hydroxypropyltrimethyl ammonium chloride chitosan Immunostimulatory activities Nanoparticles
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2021.03.148
通讯作者Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要In this study, we generated chitosan nanoparticles by exploiting the electrostatic interactions between positively charged hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and negatively charged carboxymethyl chitosan (CMC), and examined the effects of altering the molecular weight and carboxymethyl substitution sites of the chitosan molecules. Particle size, potential, and encapsulation efficiency of the various chitosan nano particles were examined; the particle size range was 162.40 & ndash;332.80 nm, the charge range was 19.50 & ndash;40.60 mV, and the encapsulation efficiency range was 48.4 & ndash;70.7%. We then examined the immunostimulatory effects of the nanoparticle variants on dendritic cells (DCs); we found that the site of carboxymethyl substitution significantly affected the immunostimulatory effects of the nanoparticles. Two nanoparticle types, 200 kDa N,O-carboxymethyl chitosan-HACC (NO-CMC-HACC) and N-carboxymethyl chitosan-HACC (N-CMC-HACC), greatly promoted the expression of interleukin-6, tumor necrosis factor, and interleukin-1 beta in DCs. Moreover, NO-CMC-HACC nanoparticles caused an increase in major histocompatibility complex-II (MHC-II), CD11c, CD80, and CD86 secretion in DCs, indicating that these nanoparticles promoted antigen presentation. We then examined chitosan nanoparticle uptake by DCs using laser confocal microscopy; we found that the NO-CMC-HACC nanoparticles were more readily absorbed by DCs compared to the N-CMC-HACC nanoparticles. Therefore, we concluded that 200 kDa NO-CMCHACC nanoparticles exhibited strong potential as immunological adjuvants. (c) 2021 Elsevier B.V. All rights reserved.
资助项目National Key Research and Development Program of China[2018YFC0311300] ; Key Research and Development Program of Shandong Province[2019GHY112015] ; Key Research and Development Program of Shandong Province[2019YYSP028]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000656912700007
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/176843]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Pengcheng
作者单位1.Chinese Acad Sci, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Megasci, Qingdao, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Chaojie,Xing, Ronge,Liu, Song,et al. The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,181:398-409.
APA Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2021).The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,181,398-409.
MLA Xu, Chaojie,et al."The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 181(2021):398-409.

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来源：海洋研究所

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