Identification of differentially expressed proteins involved in the early larval development of the Pacific oyster Crassostrea gigas
文献类型：期刊论文
| 作者 | Huan, Pin1 ; Wang, Hongxia1; Dong, Bo1,2; Liu, Baozhong1
|
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2012-07-16 |
| 卷号 | 75期号:13页码:3855-3865 |
| 关键词 | Crassostrea gigas Larval development Comparative proteomic analysis Quality control proteins Cell proliferation |
| ISSN号 | 1874-3919 |
| 通讯作者 | Liu, BZ (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. |
| 英文摘要 | The trochophore and D-veliger are two pivotal but distinct developmental stages in bivalve larval development. Complex morphological and physiological processes are involved in the transition between these stages, yet the underlying molecular mechanisms remain largely elusive. In this study, a comparative proteomic analysis was conducted to identify the proteins related to early larval development of the Pacific oyster Crassostrea gigas. Fifty proteins showing different levels of expression were screened. These spots were further analyzed using LC-MS/MS and twenty of them were identified. Together with information from Western blotting and real-time PCR assay of some annotated genes, our results provide insight into novel aspects of cellular processes and protein function in oyster larvae. For example, it was shown that cell division was unexpectedly slowed down in D-veliger larvae, while substantial gene transcription and biochemical activity were revealed. "Quality control" proteins were identified for the first time to be involved in mollusk larval development. Bivalve larval development provides an elegant system for studying cellular processes and tissue morphogenesis. Our data shed light on the molecular mechanisms underlying these complex processes and regulatory networks. (c) 2012 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemical Research Methods |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.jprot.2012.04.051 |
| 语种 | 英语 |
| WOS记录号 | WOS:000307025100005 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12349]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Riken Ctr Dev Biol, Lab Morphogenet Signaling, Kobe, Hyogo 6500047, Japan |
| 推荐引用方式 GB/T 7714 | Huan, Pin,Wang, Hongxia,Dong, Bo,et al. Identification of differentially expressed proteins involved in the early larval development of the Pacific oyster Crassostrea gigas[J]. JOURNAL OF PROTEOMICS,2012,75(13):3855-3865. |
| APA | Huan, Pin,Wang, Hongxia,Dong, Bo,&Liu, Baozhong.(2012).Identification of differentially expressed proteins involved in the early larval development of the Pacific oyster Crassostrea gigas.JOURNAL OF PROTEOMICS,75(13),3855-3865. |
| MLA | Huan, Pin,et al."Identification of differentially expressed proteins involved in the early larval development of the Pacific oyster Crassostrea gigas".JOURNAL OF PROTEOMICS 75.13(2012):3855-3865. |
入库方式： OAI收割
来源：海洋研究所
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