中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 实验海洋生物学重点实验室
Analysis of a sex-biased sequence in sex determination region and exploitation of a fast sex detection method in Litopenaeus vannamei

文献类型：期刊论文

作者Wang, Yue1,2,3; Yu, Yang1,2,4; Li, Shihao1,2,4; Zhang, Xiaojun1,2,4; Li, Fuhua1,2,4,5
刊名AQUACULTURE
出版日期2021-10-15
卷号543页码:6
关键词Sex determination region Amplification difference Sequence difference Genetic sex detection Litopenaeus vannamei
ISSN号0044-8486
DOI10.1016/j.aquaculture.2021.736922
通讯作者Yu, Yang(yuyang@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
英文摘要In order to understand the characteristics of the sex determination region in Litopenaeus vannamei, genome walking was performed based on the existing sex-linked Marker19299. A fragment of 2630 bp sequence was obtained for the genomic DNA of females, which contains two contigs, contig 122401 and contig 4497889. On contig 122401, the sequence showed a sex-biased amplification between females and males, the target band for males was much weaker than that for females. Copy number analysis showed that the female DNA contained more copies of contig 122401 sequence than that of males. Besides, the females contained an extra 9 bp insertion and a 205 bp repeat on contig 4497889 in comparison with males. These results not only provide further evidence for the ZZ/ZW sex determination system in Litopenaeus vannamei, but also suggest a novel hypothesis that the sex determination in L.vannamei might be controlled by the existence of the differences both in DNA sequence and genome structure. According to the above characteristics, a fast detection method to distinguish genetic sex of L.vannamei was developed. This research will not only provide useful information for the fine mapping of the sex determination region in L.vannamei, but also accelerate studies about the sex determination mechanism in shrimp.
资助项目Joint NSFC-ISF Research Program[31861143047] ; National Key R&D Program of China[2018YFD0900202] ; China Agriculture Research System-48 (CARS-48)
WOS研究方向Fisheries ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:000672589700012
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/177020]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Yu, Yang; Li, Fuhua
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yue,Yu, Yang,Li, Shihao,et al. Analysis of a sex-biased sequence in sex determination region and exploitation of a fast sex detection method in Litopenaeus vannamei[J]. AQUACULTURE,2021,543:6.
APA Wang, Yue,Yu, Yang,Li, Shihao,Zhang, Xiaojun,&Li, Fuhua.(2021).Analysis of a sex-biased sequence in sex determination region and exploitation of a fast sex detection method in Litopenaeus vannamei.AQUACULTURE,543,6.
MLA Wang, Yue,et al."Analysis of a sex-biased sequence in sex determination region and exploitation of a fast sex detection method in Litopenaeus vannamei".AQUACULTURE 543(2021):6.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。