A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies
文献类型：期刊论文
| 作者 | Zhang, Libo1; Yu, Hai1; Bai, Yuanyuan1; Mishra, Bijoyananda1; Yang, Xiaoxiao1; Wang, Jing1,2; Yu, Evan B.1; Li, Riyao1; Chen, Xi1 |
| 刊名 | MOLECULES
 |
| 出版日期 | 2021-10-01 |
| 卷号 | 26期号:20页码:20 |
| 关键词 | carbohydrate-protein conjugate galectin glycan-binding protein lectin multiplex assay |
| DOI | 10.3390/molecules26206194 |
| 通讯作者 | Chen, Xi(xiichen@ucdavis.edu) |
| 英文摘要 | Carbohydrate-protein conjugates have diverse applications. They have been used clinically as vaccines against bacterial infection and have been developed for high-throughput assays to elucidate the ligand specificities of glycan-binding proteins (GBPs) and antibodies. Here, we report an effective process that combines highly efficient chemoenzymatic synthesis of carbohydrates, production of carbohydrate-bovine serum albumin (glycan-BSA) conjugates using a squarate linker, and convenient immobilization of the resulting neoglycoproteins on carboxylate-coated fluorescent magnetic beads for the development of a suspension multiplex array platform. A glycan-BSA-bead array containing BSA and 50 glycan-BSA conjugates with tuned glycan valency was generated. The binding profiles of six plant lectins with binding preference towards Gal and/or GalNAc, as well as human galectin-3 and galectin-8, were readily obtained. Our results provide useful information to understand the multivalent glycan-binding properties of human galectins. The neoglycoprotein-immobilized fluorescent magnetic bead suspension multiplex array is a robust and flexible platform for rapid analysis of glycan and GBP interactions and will find broad applications. |
| 资助项目 | United States National Institutes of health (NIH)[R01AI130684] ; United States NIH Shared Instrumentation Grant[S10OD025271] ; United States NIH Shared Instrumentation Grant[S10OD018913] ; United States National Science Foundation[DBI-0722538] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000713991300001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/177143]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Chen, Xi |
| 作者单位 | 1.Univ Calif Davis, Dept Chem, Davis, CA 95616 USA 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Libo,Yu, Hai,Bai, Yuanyuan,et al. A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies[J]. MOLECULES,2021,26(20):20. |
| APA | Zhang, Libo.,Yu, Hai.,Bai, Yuanyuan.,Mishra, Bijoyananda.,Yang, Xiaoxiao.,...&Chen, Xi.(2021).A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies.MOLECULES,26(20),20. |
| MLA | Zhang, Libo,et al."A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies".MOLECULES 26.20(2021):20. |
入库方式： OAI收割
来源：海洋研究所
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