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Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I

文献类型：期刊论文

作者Liu, Ge1,2,3,4,5; Liu, Rui1,2,3,4,5; Shan, Yeqi1,2,3,4,5,6; Sun, Chaomin1,2,3,4,5,6
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
出版日期2021-10-01
卷号297期号:4页码:12
DOI10.1016/j.jbc.2021.101133
通讯作者Sun, Chaomin(sunchaomin@qdio.ac.cn)
英文摘要Many natural polysaccharides have significant anticancer activity with low toxicity, but the complex chemical structures make in-depth studies of the involved mechanisms extremely difficult. The purpose of this study was to investigate the effect of the marine bacterial exopolysaccharide (exopolysaccharide 11 [EPS11]) on liver cancer metastasis to explore the underly-ing target protein and molecular mechanism. We found that EPS11 significantly suppressed cell adhesion, migration, and invasion in liver cancer cells. Proteomic analysis showed that EPS11 induced downregulation of proteins related to the extracellular matrix-receptor interaction signaling pathway. In addition, the direct pharmacological target of EPS11 was identified as collagen I using cellular thermal shift assays. Surface plasmon resonance and pull-down assays further confirmed the specific binding of EPS11 to collagen I. More-over, EPS11 was shown to inhibit tumor metastasis by directly modulating collagen I activity via the beta 1-integrin-mediated signaling pathway. Collectively, our study demonstrated for the first time that collagen I could be a direct pharmacological target of polysaccharide drugs. Moreover, directly targeting collagen I may be a promising strategy for finding novel carbohydrate-based drugs.
资助项目National Natural Science Foundation of China[42006081] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; National Key R and D Program of China[2018YFC0310800] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Natural Science Foundation of Shandong Province[ZR2019BH024] ; Basic Applied Research program of Qingdao[19-6-2-35-cg]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000713100000012
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/177194]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_深海极端环境与生命过程研究中心
通讯作者Sun, Chaomin
作者单位1.Chinese Acad Sci, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Ge,Liu, Rui,Shan, Yeqi,et al. Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2021,297(4):12.
APA Liu, Ge,Liu, Rui,Shan, Yeqi,&Sun, Chaomin.(2021).Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I.JOURNAL OF BIOLOGICAL CHEMISTRY,297(4),12.
MLA Liu, Ge,et al."Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I".JOURNAL OF BIOLOGICAL CHEMISTRY 297.4(2021):12.

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来源：海洋研究所

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