Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System
文献类型:期刊论文
作者 | Tan Xungang1,2; Wang Ling1,2,3; Wu Zhihao1,2; Jiao Shuang1,2; Wang Lijuan1,2; Zou Yuxia1,2; Jiang Jingteng4,5; You Feng1,2 |
刊名 | JOURNAL OF OCEAN UNIVERSITY OF CHINA |
出版日期 | 2021-12-01 |
卷号 | 20期号:6页码:1528-1536 |
ISSN号 | 1672-5182 |
关键词 | CRISPR Cas9 gsdf microinjection myomaker needle with brake olive flounder |
DOI | 10.1007/s11802-021-4772-6 |
通讯作者 | Tan Xungang(tanx@qdio.ac.cn) ; You Feng(youfeng@qdio.ac.cn) |
英文摘要 | The whole-genome sequence of the olive flounder (Paralichthys olivaceus) provides a basis for gene functional analyses, which is important for the aquaculture industry. Understanding gene function will help us to select better economic traits such as fast growth and better culture conditions, which further will increase the aquaculture output. Gene knockout is an important reverse genetics approach for in vivo studies of gene function. In this study, the CRISPR/Cas9 genome editing method with a microinjection system using a simple braked needle was employed in olive flounder. After injection in embryos, green fluorescent protein expression was detected in 40% of larvae. The proportion of normal-hatched larvae was approximately 50%. Different mutations, including short indels and fragment deletions, were found in our test genes gsdf and myomaker. Additionally, we detected more than one mutation in a single larva. In summary, our microinjection technique and CRISPR/Cas9 can be applied to study gene functions in olive flounder. |
WOS研究方向 | Oceanography |
语种 | 英语 |
出版者 | OCEAN UNIV CHINA |
WOS记录号 | WOS:000720813700021 |
源URL | [http://ir.qdio.ac.cn/handle/337002/177212] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
通讯作者 | Tan Xungang; You Feng |
作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab ForMarine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Fishery Technol Promot Stn, Weihai 264200, Peoples R China 5.Shenghang Aquat Sci & Technol Co Ltd, Weihai 264200, Peoples R China |
推荐引用方式 GB/T 7714 | Tan Xungang,Wang Ling,Wu Zhihao,et al. Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2021,20(6):1528-1536. |
APA | Tan Xungang.,Wang Ling.,Wu Zhihao.,Jiao Shuang.,Wang Lijuan.,...&You Feng.(2021).Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System.JOURNAL OF OCEAN UNIVERSITY OF CHINA,20(6),1528-1536. |
MLA | Tan Xungang,et al."Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System".JOURNAL OF OCEAN UNIVERSITY OF CHINA 20.6(2021):1528-1536. |
入库方式: OAI收割
来源:海洋研究所
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