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Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome

文献类型：期刊论文

作者Meng, Jie1,2,3; Zhang, Guofan3,4; Wang, Wen-Xiong1,2,5
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2022
卷号120页码:202-213
ISSN号1050-4648
关键词Oyster scRNA-seq Granulocytes Hematopoietic factor Pseudo-temporal ordering
DOI10.1016/j.fsi.2021.11.027
通讯作者Zhang, Guofan(gfzhang@qdio.ac.cn) ; Wang, Wen-Xiong(wx.wang@cityu.edu.hk)
英文摘要Oyster is the worldwide aquaculture molluscan and evolves a complex immune defense system, with hemocytes as the major immune system for its host defense. However, the functional heterogeneity of hemocyte has not been characterized, which markedly hinders our understanding of its defense role. Here, we used the single-cell transcriptome profiling (scRNA-seq), which provides a high-resolution visual insight into its dynamics, to map the hemocyte and assess its heterogeneity in a molluscan oyster Crassostrea hongkongensis. By combining with the cell type specific RNA-seq, thirteen subpopulations belonging to granulocyte, semi-granulocyte, and hyalinocyte were revealed. The granulocytes mainly participated in immune response and autophagy process. Pseudotemporal ordering of granulocytes identified two different cell-lineages. The hematopoietic transcription factors regulated networks controlling their differentiations were also identified. We further identified one subpopulation of granulocytes in immune activate states with the cell cycle and immune responsive genes expressions, which illustrated the functional heterogeneity of the same cell type. Collectively, our scRNA-seq analysis demonstrated the hemocytes diversity of molluscans. The results are important in our understanding of the immune defense evolution and functional differentiation of hemocytes in Phylum Mollusca.
资助项目Youth Innovation Promotion Asso-ciation of the Chinese Academy of Science[2019210]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号WOS:000724951600002
源URL[http://ir.qdio.ac.cn/handle/337002/177290]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Zhang, Guofan; Wang, Wen-Xiong
作者单位1.City Univ Hong Kong, Sch Energy & Environm, Kowloon, Hong Kong, Peoples R China
2.City Univ Hong Kong, Hong Kong Branch Southern Marine Sci & Engn, State Key Lab Marine Pollut, Guangdong Lab Guangzhou,Kowloon, Hong Kong, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
5.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
推荐引用方式
GB/T 7714		 Meng, Jie,Zhang, Guofan,Wang, Wen-Xiong. Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome[J]. FISH & SHELLFISH IMMUNOLOGY,2022,120:202-213.
APA Meng, Jie,Zhang, Guofan,&Wang, Wen-Xiong.(2022).Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome.FISH & SHELLFISH IMMUNOLOGY,120,202-213.
MLA Meng, Jie,et al."Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome".FISH & SHELLFISH IMMUNOLOGY 120(2022):202-213.

入库方式： OAI收割

来源：海洋研究所

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