Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis
文献类型：期刊论文
| 作者 | Li, Shuang1,2,3 ; Shao, Zhanru1,2; Lu, Chang1,2,3; Yao, Jianting1,2; Zhou, Yongdong4; Duan, Delin1,2
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| 刊名 | MOLECULES
 |
| 出版日期 | 2021-11-01 |
| 卷号 | 26期号:22页码:16 |
| 关键词 | heterologous expression protein purification enzyme activity assay site-directed mutagenesis abiotic stresses |
| DOI | 10.3390/molecules26226793 |
| 通讯作者 | Shao, Zhanru(zrshao@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn) |
| 英文摘要 | Pyropia haitanensis is an important laver species in China. Its quality traits are closely related to the content of glutamic acid. Glutamate dehydrogenase (GDH) is a crucial enzyme in the glutamic acid metabolism. In this study, two GDH genes from P. haitanensis, PhGDH1 and PhGDH2, were cloned and successfully expressed in Escherichia coli. The in vitro enzyme activity assay demonstrated that the catalytic activity of PhGDHs is mainly in the direction of ammonium assimilation. The measured K-m values of PhGDH1 for NADH, (NH4)(2)SO4, and alpha-oxoglutarate were 0.12, 4.99, and 0.16 mM, respectively, while the corresponding K-m values of PhGDH2 were 0.02, 3.98, and 0.104 mM, respectively. Site-directed mutagenesis results showed that Gly(193) and Thr(361) were important catalytic residues for PhGDH2. Moreover, expression levels of both PhGDHs were significantly increased under abiotic stresses. These results suggest that PhGDHs can convert alpha-oxoglutarate to glutamic acid, and enhance the flavor and stress resistance of P. haitanensis. |
| 资助项目 | National Key R&D Program of China[2018YFD0900106] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000725718000001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/177293]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Shao, Zhanru; Duan, Delin |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yancheng Hairui Food Co Ltd, Dafeng 224005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shuang,Shao, Zhanru,Lu, Chang,et al. Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis[J]. MOLECULES,2021,26(22):16. |
| APA | Li, Shuang,Shao, Zhanru,Lu, Chang,Yao, Jianting,Zhou, Yongdong,&Duan, Delin.(2021).Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis.MOLECULES,26(22),16. |
| MLA | Li, Shuang,et al."Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis".MOLECULES 26.22(2021):16. |
入库方式： OAI收割
来源：海洋研究所
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