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Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons

文献类型：期刊论文

作者Sun, Yan1,2,3,4; Wang, Minxiao1,2,3,4; Zhong, Zhaoshan1,2,3,4; Chen, Hao1,2,3,4; Wang, Hao1,2,3,4; Zhou, Li1,2,3,4; Cao, Lei1,2,3,4; Fu, Lulu1,2,3,4; Zhang, Huan1,2,3,4; Lian, Chao1,2,3,4
刊名SCIENCE OF THE TOTAL ENVIRONMENT
出版日期2022-01-15
卷号804页码:13
关键词Sulfide detoxification Deep-sea adaptation Bathymodiolinae mussels Sulfide:quinone oxidoreductase Symbionts Mitochondria
ISSN号0048-9697
DOI10.1016/j.scitotenv.2021.150054
通讯作者Sun, Song(sunsong@qdio.ac.cn) ; Li, Chaolun(lcl@qdio.ac.cn)
英文摘要The deep-sea mussel Gigantidas plantifrons is a representative species that relies on nutrition provided by chemoautotrophic endosymbiotic bacteria to survive in both hydrothermal vent and methane seep environments. However, vent and seep habitats have distinct geochemical features, with vents being more harsh than seeps because of abundant toxic chemical substances, particularly hydrogen sulfide (H2S). Until now, the adaptive strategies of G. platifrons in a heterogeneous environment and their sulfide detoxification mechanisms are still unclear. Herein, we conducted 16S rDNA sequencing and metatranscriptome sequencing of G. plan:trans collected from a methane seep at Formosa Ridge in the South China Sea and a hydrothermal vent at lheya North Knoll in the Mid-Okinawa Trough to provide a model for understanding environmental adaption and sulfide detoxification mechanisms, and a three-day laboratory controlled Na2S stress experiment to test the transcriptomic responses under sulfide stress. The results revealed the active detoxification of sulfide in G. platifrons gills. First, epibiotic Campylobacterota bacteria were more abundant in vent mussels and contributed to environmental adaptation by active oxidation of extracellular H2S. Notably, a key sulfide-oxidizing gene, sulfide:quinone oxidoreductase (sqr), derived from the methanotrophic endosymbiont, was significantly upregulated in vent mussels, indicating the oxidization of intracellular sulfide by the endosymbiont. In addition, transcriptomic comparison further suggested that genes involved in oxidative phosphorylation and mitochondrial sulfide oxidization pathway played important roles in the sulfide tolerance of the host mussels. Moreover, uanscriptomic analysis of Na2S stressed mussels confirmed the upregulation of oxidative phosphorylation and sulfide oxidization genes in response to sulfide exposure. Overall, this study provided a systematic transcriptional analysis of both the active bacterial community members and the host mussels, suggesting that the epibionts, endosymbionts, and mussel host collaborated on sulfide detoxification from extracellular to intracellular space to adapt to harsh H2S-rich environments. (C) 2021 Published by Elsevier B.V.
资助项目National Key Research and Development Program of China[2018YFC0310800] ; National Key Research and Development Program of China[2017YFC1404401] ; National Natural Science Foundation of China[42030407] ; National Natural Science Foundation of China[42076091] ; National Natural Science Foundation of China[41806200] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Open Research Project of National Major Science & Technology Infrastructure[NMSTI-KEXUE2017K01] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2021GH01] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2019GZ02] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2019GZ06] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2018G16]
WOS研究方向Environmental Sciences & Ecology
语种英语
WOS记录号WOS:000701855500014
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/176491]  
专题海洋研究所_海洋生态与环境科学重点实验室
通讯作者Sun, Song; Li, Chaolun
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Yan,Wang, Minxiao,Zhong, Zhaoshan,et al. Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,804:13.
APA Sun, Yan.,Wang, Minxiao.,Zhong, Zhaoshan.,Chen, Hao.,Wang, Hao.,...&Li, Chaolun.(2022).Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons.SCIENCE OF THE TOTAL ENVIRONMENT,804,13.
MLA Sun, Yan,et al."Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons".SCIENCE OF THE TOTAL ENVIRONMENT 804(2022):13.

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来源：海洋研究所

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