Novel Non-paralytic Shellfish Toxin and Non-spirolide Toxicity to Finfish, Brine Shrimp, and Rotifer Observed in a Culture of the Dinoflagellate Alexandrium insuetum Isolated From the Coastal Water of China
文献类型：期刊论文
| 作者 | Song, Xiaoying1,2,3,4 ; Zhai, Xinyu1,3; Hao, Shuang5; Shang, Lixia1,2,4; Deng, Yunyan1,2,4; Chai, Zhaoyang1,2,4; Chen, Junhui5; Hu, Zhangxi1,2,4; Tang, Ying Zhong1,2,4
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| 刊名 | FRONTIERS IN MARINE SCIENCE
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| 出版日期 | 2021-09-28 |
| 卷号 | 8页码:17 |
| 关键词 | harmful algal blooms (HABs) Alexandrium insuetum morphology phylogeny novel toxicity fish kill |
| DOI | 10.3389/fmars.2021.735752 |
| 通讯作者 | Hu, Zhangxi(zhu@qdio.ac.cn) ; Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn) |
| 英文摘要 | The genus Alexandrium is one of the major harmful algal blooms (HABs)-forming dinoflagellate group and at least half of ~40 described species have been reported to produce paralytic shellfish toxins (PSTs). The potentially harmful species Alexandrium insuetum has been reported from many countries of Asia and Europe, and to have paralytic shellfish poisoning toxicity, but no mortality of marine animals was observed during its bloom. Therefore, it is ecologically important to characterize the possible toxicity and toxins of this organism. In this study, based on the establishment of two clonal cultures through cyst germination collected from the Yellow Sea, we identified A. insuetum from China as the first record via light microscopy (LM) and scanning electron microscopy (SEM) observations and phylogenetic analyses. The cultures of A. insuetum were further observed to be toxic to finfish and zooplankton and deleterious to rotifer eggs via laboratory bioassays. The exposure bioassays using rotifer (Brachionus plicatilis), brine shrimp (Artemia salina), and larval finfish (Oryzias melastigma) demonstrated that A. insuetum caused significant lethal effects on finfish and zooplankton species. Rotifer bioassays using cell-free culture medium, heat-treated cultures, and water, methanol, and trichloromethane extracts of algal cells revealed that A. insuetum produced heat-labile, water-soluble toxin(s) that could be excreted from A. insuetum cells and steadily accumulated in the medium during the growth phases. Hatching success of rotifer eggs was also found to be seriously affected by the exposure to A. insuetum. Importantly, ultra-high performance liquid chromatography-tandem mass spectrometry [UPLC (or LC)-MS-MS] analyses suggest the above-described toxicity of A. insuetum was caused by neither PSTs nor spiroimines (13-desmethyl spirolide C and gymnodimine). Collectively, our findings demonstrated the novel toxicity to finfish and zooplankton in A. insuetum, which is ecologically important in not only possibly contributing to population dynamics and even the formation of HABs of the species, but also affecting the on-the-spot survival and the reproduction potency of marine animals. The present work is believed to set a cornerstone for the monitoring and risk assessment of the species along the coastal waters of China and for understanding the general ecology of A. insuetum. |
| 资助项目 | Science & Technology Basic Resources Investigation Program of China[2018FY100200] ; National Science Foundation of China[41976134] ; National Science Foundation of China[41776125] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q09] |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000706070100001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/176672]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Hu, Zhangxi; Tang, Ying Zhong |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Minist Nat Resources, Inst Oceanog 1, Key Lab Sci & Engn Marine Ecol & Environm, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Xiaoying,Zhai, Xinyu,Hao, Shuang,et al. Novel Non-paralytic Shellfish Toxin and Non-spirolide Toxicity to Finfish, Brine Shrimp, and Rotifer Observed in a Culture of the Dinoflagellate Alexandrium insuetum Isolated From the Coastal Water of China[J]. FRONTIERS IN MARINE SCIENCE,2021,8:17. |
| APA | Song, Xiaoying.,Zhai, Xinyu.,Hao, Shuang.,Shang, Lixia.,Deng, Yunyan.,...&Tang, Ying Zhong.(2021).Novel Non-paralytic Shellfish Toxin and Non-spirolide Toxicity to Finfish, Brine Shrimp, and Rotifer Observed in a Culture of the Dinoflagellate Alexandrium insuetum Isolated From the Coastal Water of China.FRONTIERS IN MARINE SCIENCE,8,17. |
| MLA | Song, Xiaoying,et al."Novel Non-paralytic Shellfish Toxin and Non-spirolide Toxicity to Finfish, Brine Shrimp, and Rotifer Observed in a Culture of the Dinoflagellate Alexandrium insuetum Isolated From the Coastal Water of China".FRONTIERS IN MARINE SCIENCE 8(2021):17. |
入库方式： OAI收割
来源：海洋研究所
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