Japanese flounder (Paralichthys olivaceus) Hsp70: Adjuvant effect and its dependence on the intrinsic ATPase activity
文献类型：期刊论文
| 作者 | Hu, Yong-hua1; Dang, Wei1,2,3; Zhang, Min1; Sun, Li1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 33期号:4页码:829-834 |
| 关键词 | Paralichthys olivaceus Heat shock protein 70 Adjuvant Vaccine |
| ISSN号 | 1050-4648 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Heat shock protein (Hsp) 70 is a molecular chaperone that plays an important role in protein folding and transport. In addition, Hsp70 is also involved in regulation of innate and adaptive immune response. In this study, we examined the biological activity and the immunomodulatory property of an Hsp70 homologue, PoHsp70, from Japanese flounder (Paralichthys olivaceus). Recombinant PoHsp70 purified from Escherichia coli exhibits apparent ATPase activity; however, a mutant PoHsp70, PoHsp70M, that bears mutation of the ATPase-associated domain, was completely abolished in activity. Expression of PoHsp70 was upregulated in a time-dependent manner by vaccination of flounder with a DNA vaccine, pSia10. that expresses a Streptococcus iniae antigen, Sia10. To examine whether PoHsp70 possessed any adjuvant potential, the DNA vaccine plasmids pSia10Hsp70 and pSia10Hsp70M were constructed. pSia10Hsp70 co-expresses Sia10 and PoHsp70, while pSia10Hsp70M co-expresses Sia10 and PoHsp70M. Following vaccination of flounder, production of Sia10 plus PoHsp70 and Sia10 plus PoHsp70M was detected in pSia10Hsp70- and pSia10Hsp70M-vaccinated fish respectively. At one month post-vaccination, comparable levels of serum antibodies were detected in fish vaccinated with pSia10Hsp70, pSia10Hsp70M, and pSia10. Subsequent protection analysis showed that, followings. iniae challenge, pSia10Hsp70 induced a survival rate that was significantly higher than that induced by pSia10, while pSia10Hsp70M induced a survival rate similar to that induced by pSia10. These results indicate that PoHsp70 is an effective adjuvant and that the adjuvanticity of PoHsp70 requires the intrinsic ATPase activity. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2012.07.015 |
| 语种 | 英语 |
| WOS记录号 | WOS:000310185800019 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12357]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou Key Lab Anim Adaptat & Evolut, Hangzhou 310036, Zhejiang, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Yong-hua,Dang, Wei,Zhang, Min,et al. Japanese flounder (Paralichthys olivaceus) Hsp70: Adjuvant effect and its dependence on the intrinsic ATPase activity[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(4):829-834. |
| APA | Hu, Yong-hua,Dang, Wei,Zhang, Min,&Sun, Li.(2012).Japanese flounder (Paralichthys olivaceus) Hsp70: Adjuvant effect and its dependence on the intrinsic ATPase activity.FISH & SHELLFISH IMMUNOLOGY,33(4),829-834. |
| MLA | Hu, Yong-hua,et al."Japanese flounder (Paralichthys olivaceus) Hsp70: Adjuvant effect and its dependence on the intrinsic ATPase activity".FISH & SHELLFISH IMMUNOLOGY 33.4(2012):829-834. |
入库方式： OAI收割
来源：海洋研究所
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