Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications
文献类型：期刊论文
| 作者 | Song, Huiyin1,2,3; Chen, Yang1,2,3,4 ; Liu, Feng1,2,3; Chen, Nansheng1,2,3,5
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| 刊名 | FRONTIERS IN MICROBIOLOGY
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| 出版日期 | 2021-07-02 |
| 卷号 | 12页码:18 |
| 关键词 | mtDNA sizes mitochondrial genomes Phaeocystis globosa repeat region Haptophyta |
| DOI | 10.3389/fmicb.2021.676447 |
| 通讯作者 | Chen, Nansheng(chenn@qdio.ac.cn) |
| 英文摘要 | The haptophyte Phaeocystis globosa is a well-known species for its pivotal role in global carbon and sulfur cycles and for its capability of forming harmful algal blooms (HABs) with serious ecological consequences. Its mitochondrial genome (mtDNA) sequence has been reported in 2014 but it remains incomplete due to its long repeat sequences. In this study, we constructed the first full-length mtDNA of P. globosa, which was a circular genome with a size of 43,585 bp by applying the PacBio single molecular sequencing method. The mtDNA of this P. globosa strain (CNS00066), which was isolated from the Beibu Gulf, China, encoded 19 protein-coding genes (PCGs), 25 tRNA genes, and two rRNA genes. It contained two large repeat regions of 6.7 kb and similar to 14.0 kb in length, respectively. The combined length of these two repeat regions, which were missing from the previous mtDNA assembly, accounted for almost half of the entire mtDNA and represented the longest repeat region among all sequenced haptophyte mtDNAs. In this study, we tested the hypothesis that repeat unit amplification is a driving force for different mtDNA sizes. Comparative analysis of mtDNAs of five additional P. globosa strains (four strains obtained in this study, and one strain previously published) revealed that all six mtDNAs shared identical numbers of genes but with dramatically different repeat regions. A homologous repeat unit was identified but with hugely different numbers of copies in all P. globosa strains. Thus, repeat amplification may represent an important driving force of mtDNA evolution in P. globosa. |
| 资助项目 | National Key Research Development Program of China[2017YFC1404302] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan (Talent Development Program -5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16zhc] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0504] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000674041600001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/177005]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, Nansheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Sch Earth & Planetary, Beijing, Peoples R China 5.Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC, Canada |
| 推荐引用方式 GB/T 7714 | Song, Huiyin,Chen, Yang,Liu, Feng,et al. Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications[J]. FRONTIERS IN MICROBIOLOGY,2021,12:18. |
| APA | Song, Huiyin,Chen, Yang,Liu, Feng,&Chen, Nansheng.(2021).Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications.FRONTIERS IN MICROBIOLOGY,12,18. |
| MLA | Song, Huiyin,et al."Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications".FRONTIERS IN MICROBIOLOGY 12(2021):18. |
入库方式： OAI收割
来源：海洋研究所
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