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Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5

文献类型：期刊论文

作者Cai Jinling2; Wei Ying2; Zhao Yupeng2; Pan Guanghua2; Wang Guangce1
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2012-07-01
卷号30期号:4页码:620-626
关键词polyhydroxybutyrate Rhodovulum sulfidophilum nitrogen source carbon source carbon to nitrogen molar ratio
ISSN号0254-4059
通讯作者Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
英文摘要The effects of different NaCl concentrations, nitrogen sources, carbon sources, and carbon to nitrogen molar ratios on biomass accumulation and polyhydroxybutyrate (PHB) production were studied in batch cultures of the marine photosynthetic bacterium Rhodovulum sulfidophilum P5 under aerobic-dark conditions. The results show that the accumulation of PHB in strain P5 is a growth-associated process. Strain P5 had maximum biomass and PHB accumulation at 2%-3% NaCl, suggesting that the bacterium can maintain growth and potentially produce PHB at natural seawater salinity. In the nitrogen source test, the maximum biomass accumulation (8.10 +/- 0.09 g/L) and PHB production (1.11 +/- 0.13 g/L and 14.62%+/- 2.2 of the cell dry weight) were observed when peptone and ammonium chloride were used as the sole nitrogen source. NH (4) (+) -N was better for PHB production than other nitrogen sources. In the carbon source test, the maximum biomass concentration (7.65 +/- 0.05 g/L) was obtained with malic acid as the sole carbon source, whereas the maximum yield of PHB (5.03 +/- 0.18 g/L and 66.93%+/- 1.69% of the cell dry weight) was obtained with sodium pyruvate as the sole carbon source. In the carbon to nitrogen ratios test, sodium pyruvate and ammonium chloride were selected as the carbon and nitrogen sources, respectively. The best carbon to nitrogen molar ratio for biomass accumulation (8.77 +/- 0.58 g/L) and PHB production (6.07 +/- 0.25 g/L and 69.25%+/- 2.05% of the cell dry weight) was 25. The results provide valuable data on the production of PHB by R. sulfidophilum P5 and further studies are on-going for best cell growth and PHB yield.
学科主题Limnology ; Oceanography
收录类别SCI
原文出处10.1007/s00343-012-1132-9
语种英语
WOS记录号WOS:000305906700014
公开日期2013-09-24
源URL[http://ir.qdio.ac.cn/handle/337002/12372]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China
推荐引用方式
GB/T 7714		 Cai Jinling,Wei Ying,Zhao Yupeng,et al. Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(4):620-626.
APA Cai Jinling,Wei Ying,Zhao Yupeng,Pan Guanghua,&Wang Guangce.(2012).Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(4),620-626.
MLA Cai Jinling,et al."Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.4(2012):620-626.

入库方式： OAI收割

来源：海洋研究所

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