RNAi assay in primary cells: a new method for gene function analysis in marine bivalve
文献类型：期刊论文
| 作者 | You, Yanan1,2; Huan, Pin1 ; Liu, Baozhong1
|
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 39期号:8页码:8209-8216 |
| 关键词 | Marine bivalve Primary cell culture RNAi assay Gene function analysis |
| ISSN号 | 0301-4851 |
| 通讯作者 | Liu, BZ (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 英文摘要 | RNA interference (RNAi) is an effective approach for gene function analysis, which is well developed in mammal cell lines. However, RNAi has rarely been reported in marine bivalve species. To provide support on functional analysis of bivalve genes, for the first time to our knowledge, we conducted RNAi assay on primary cell of clam Meretrix meretrix in this study. Firstly we explored the method of culturing primary cells of M. meretrix to ensure the cells to live at high activity for at least 2 weeks. Ferritin gene was chosen as the target gene and RNAi assay was conducted through soaking the primary cells of M. meretrix digestive gland in medium containing dsRNA of ferritin gene. Realtime PCR, western blot and immunocytochemistry analysis were used to analyze the inhibition of gene expression after RNAi. Results showed the ferritin mRNA was significantly down-regulated by 66.11% after RNAi. Western blot result showed that the expression level of ferritin protein was also depressed post RNAi. The method developed in this study proved to be reliable and effective for RNAi assay on marine bivalve cells. It would be an efficient tool for gene function analysis in marine bivalves and more studies based on primary cells of marine bivalves can be expected. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s11033-012-1668-y |
| 语种 | 英语 |
| WOS记录号 | WOS:000305749200041 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12375]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Yanan,Huan, Pin,Liu, Baozhong. RNAi assay in primary cells: a new method for gene function analysis in marine bivalve[J]. MOLECULAR BIOLOGY REPORTS,2012,39(8):8209-8216. |
| APA | You, Yanan,Huan, Pin,&Liu, Baozhong.(2012).RNAi assay in primary cells: a new method for gene function analysis in marine bivalve.MOLECULAR BIOLOGY REPORTS,39(8),8209-8216. |
| MLA | You, Yanan,et al."RNAi assay in primary cells: a new method for gene function analysis in marine bivalve".MOLECULAR BIOLOGY REPORTS 39.8(2012):8209-8216. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。