Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)
文献类型：期刊论文
| 作者 | Jin, Weihua1,2; Wang, Jing1 ; Ren, Sumei3; Song, Ni3; Zhang, Quanbin1
|
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 10期号:10页码:2138-2152 |
| 关键词 | ESI-CID-MS/MS fucoidan oligosaccharide Saccharina japonica |
| ISSN号 | 1660-3397 |
| 通讯作者 | Zhang, QB (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 英文摘要 | A fucoidan extracted from Saccharina japonica was fractionated by anion exchange chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and gel filtration chromatography while Fraction Y2 was fractionated by gel filtration chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1 -> 3 Man 1 -> 4 GlcA, Man 1 -> 3 GlcA 1 -> 4 GlcA, Fuc 1 -> 4 GlcA and Fuc 1 -> 3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6. |
| 学科主题 | Chemistry, Medicinal |
| 收录类别 | SCI |
| 原文出处 | 10.3390/md10102138 |
| 语种 | 英语 |
| WOS记录号 | WOS:000310453600001 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12386]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean Univ China, Coll Med & Pharmaceut, Qingdao 266003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, Weihua,Wang, Jing,Ren, Sumei,et al. Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)[J]. MARINE DRUGS,2012,10(10):2138-2152. |
| APA | Jin, Weihua,Wang, Jing,Ren, Sumei,Song, Ni,&Zhang, Quanbin.(2012).Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS).MARINE DRUGS,10(10),2138-2152. |
| MLA | Jin, Weihua,et al."Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)".MARINE DRUGS 10.10(2012):2138-2152. |
入库方式： OAI收割
来源：海洋研究所
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