The arginine kinase in Zhikong scallop Chlamys farreri is involved in immunomodulation
文献类型：期刊论文
| 作者 | Shi, Xiaowei1,2; Wang, Lingling1; Zhou, Zhi1,2; Yang, Chuanyan1,2; Gao, Yang1,2 ; Wang, Leilei1,2; Song, Linsheng1
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| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 出版日期 | 2012-06-01 |
| 卷号 | 37期号:2页码:270-278 |
| 关键词 | Arginine kinase (AK) Immune response Kinetic parameters L-arginine Lipopolysaccharide (LPS) Inducible nitric oxide synthase (iNOS) Nitric oxide (NO) |
| ISSN号 | 0145-305X |
| 通讯作者 | Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. |
| 英文摘要 | Arginine kinase (AK) catalyzes the reversible phosphorylation of L-arginine to form phosphoarginine, and plays a critical role in energy metabolism in invertebrates. In the present study, a scallop AK gene was identified from Chlamys farreri with an open reading frame (ORF) of 1101 bp encoding for a protein of 366 amino acids (designed as CfAK). An ATP-gua PtransN domain which was described as a guanidine substrate specificity domain (GS domain) and an ATP-gua Ptrans domian which was responsible for binding ATP, were both identified in CfAK. The mRNA transcripts of CfAK were detectable in haemocytes, hepatopancreas, adductor muscle, mantle, gill, kidney and gonad, with the highest expression level in the muscle and the lowest level in the hemocytes. The expression level of OAK mRNA increased from fertilized eggs to eyebot, and reached the highest in the trochophore stage. The relative expression level of CfAK mRNA in muscle was up-regulated significantly after LPS (0.5 mg/mL) stimulation, and reached the peak at 6 h (5.2-fold, P <0.05). The activity of inducible nitric oxide synthase (iNOS) in the supernatant of muscle homogenate increased significantly from 3.2 U/mg at Oh to 9.7 U/mg at 12 h after LPS stimulation, while the concentration of nitric oxide (NO) in the supernatant of muscle homogenate began to increase at 3 h (21.55 mu mol/L), and reached the top concentration at 24 h (42.27 mu mol/L), then recovered to the normal level after 48 h. The recombinant protein of CfAK (rCfAK) expressed in Escherichia coli displayed Arginine kinase activity, and its apparent K-m was 0.82 +/- 0.11 and 1.24 +/- 0.13 mM for L-arginine and ATP-Na, respectively. The results indicated that the CfAK was involved in energy production and utilization during the whole life process, and might refer to the immunomodulation process via altering the NO concentration and iNOS activity in scallop Chlamys farreri. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Zoology |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.dci.2012.03.008 |
| 语种 | 英语 |
| WOS记录号 | WOS:000306891400006 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12390]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Xiaowei,Wang, Lingling,Zhou, Zhi,et al. The arginine kinase in Zhikong scallop Chlamys farreri is involved in immunomodulation[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,37(2):270-278. |
| APA | Shi, Xiaowei.,Wang, Lingling.,Zhou, Zhi.,Yang, Chuanyan.,Gao, Yang.,...&Song, Linsheng.(2012).The arginine kinase in Zhikong scallop Chlamys farreri is involved in immunomodulation.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,37(2),270-278. |
| MLA | Shi, Xiaowei,et al."The arginine kinase in Zhikong scallop Chlamys farreri is involved in immunomodulation".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 37.2(2012):270-278. |
入库方式： OAI收割
来源：海洋研究所
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