中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi

文献类型:期刊论文

作者Wang, Shuangyan1,2; Cui, Zhaoxia1; Liu, Yuan1; Li, Qianqian1,2; Song, Chengwen1,2
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2012-02-01
卷号32期号:2页码:331-338
关键词Portunus trituberculatus Pacifastin mRNA expression profile Immunity
ISSN号1050-4648
通讯作者Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要Among all the serine proteinase inhibitor families (SPIs), the pacifastin-related inhibitor is seldom isolated. A pacifastin-related SPI named as PtPLC was identified from the swimming crab Portunus trituberculatus in this study. The full-length of PtPLC was cloned from haemocytes cDNA library by the combination of homology cloning and rapid amplification of cDNA ends (RACE). The PtPLC contained an open reading frame (ORF) of 1098 bp encoding a putative pacifastin-related precursor of 365 amino acids, a 5'-untranslated region (UTR) of 33 bp, and a 3'-UTR of 524 bp. The estimated molecular weight of mature PtPLC was 40.51 kDa and its isoelectric point was 5.04. Eight PLD domains in PtPLC shared a common characteristic of conserved array of six cysteine residues (Cys-Xaa(9-12)-Cys-Asn-Xaa-Cys-Xaa- Cys-Xaa(2-3)-Gly-Xaa(3-4)-Cys-Thr-Xaa(3)-Cys). The mRNA expression of PtPLC transcripts was highly detected in haemocytes, gill, hepatopancreas and stomach. The temporal expression levels of PtPLC transcripts in haemocytes showed different expression patterns after challenged by Gram-negative bacteria Vibrio alginolyticus, Gram-positive bacteria Micrococcus luteus and fungus Pichia pastoris. There were two peaks in the mRNA expression profile after M. luteus stimulation. And after V. alginolyticus and P. pastoris stimulation, there were three peaks in the mRNA expression profiles. These findings suggest that PtPLC is involved in the antibacterial defense mechanism of P. tritubercualtus. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别SCI
原文出处10.1016/j.fsi.2011.11.025
语种英语
WOS记录号WOS:000300751800012
公开日期2013-09-24
源URL[http://ir.qdio.ac.cn/handle/337002/12393]  
专题海洋研究所_海洋生物技术研发中心
海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
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GB/T 7714
Wang, Shuangyan,Cui, Zhaoxia,Liu, Yuan,et al. The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(2):331-338.
APA Wang, Shuangyan,Cui, Zhaoxia,Liu, Yuan,Li, Qianqian,&Song, Chengwen.(2012).The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi.FISH & SHELLFISH IMMUNOLOGY,32(2),331-338.
MLA Wang, Shuangyan,et al."The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi".FISH & SHELLFISH IMMUNOLOGY 32.2(2012):331-338.

入库方式: OAI收割

来源:海洋研究所

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