An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas
文献类型：期刊论文
| 作者 | Wang, Xiaotong; Song, Xiaorui ; Li, Li; Zhang, Guofan
|
| 刊名 | ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
 |
| 出版日期 | 2012 |
| 卷号 | 64页码:800 |
| 关键词 | mollusc bivalve Pacific oyster shell DNA marker-assisted selection (MAS) |
| ISSN号 | 0792-156X |
| 通讯作者 | Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | We earlier developed a method to extract DNA from in vivo-sampled mantle tissue of the bivalve Pacific oyster (Crassostrea gigas) for molecular marker-assisted selection (MAS) in breeding. However, mortality was too high when using this method. In the current study, a more efficient and safer method of extracting DNA from the oyster shell was developed, based on the improved phenol-chloroform DNA extraction method. Both nuclear DNA and mitochondrial DNA were successfully extracted from the oyster shell, and mortality was 0%. These results illustrate the safety of extracting DNA from the bivalve shell. In addition, because DNA is a cellular component, the results provide molecular evidence that cells are likely involved in shell formation. |
| 学科主题 | Fisheries |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000316859200001 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12211]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaotong,Song, Xiaorui,Li, Li,et al. An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas[J]. ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,2012,64:800. |
| APA | Wang, Xiaotong,Song, Xiaorui,Li, Li,&Zhang, Guofan.(2012).An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas.ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,64,800. |
| MLA | Wang, Xiaotong,et al."An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas".ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH 64(2012):800. |
入库方式： OAI收割
来源：海洋研究所
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