Cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye
文献类型：期刊论文
| 作者 | Li Cuiping2; Li Pengcheng1 ; Feng Jinhua1; Li Rongfeng1; Yu Huahua1
|
| 刊名 | TOXICOLOGY AND INDUSTRIAL HEALTH
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 28期号:2页码:186-192 |
| ISSN号 | 0748-2337 |
| 关键词 | Cytotoxicity jellyfish nematocysts venom Cyanea nozakii Kishinouye |
| 通讯作者 | Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | In this article, the cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye on human hepatoma cells (Bel-7402, SMMC-7721) and human colon cancer cells (H630) was investigated first. Of the three kinds of cells, the venom had the strongest cytotoxicity on H630 cells with the 50% lethal concentration (IC50) of 5.1 mu g/ml. However, the IC50 on Bel-7402 and SMMC-7721 was approximate 17.9 and 24.3 mu g/ml, respectively. The cytotoxicity of the venom was affected by pH, temperature and storage conditions. At the pH 4.5-8.5, the venom displayed obvious cytotoxicity and the percentage of survival was about 50%. When pre-incubated at temperatures over 60 degrees C for as short as 10 min, the percentage of survival sharply improved from 4.6% up to 80%. The venom was stored in a more stable condition at -80 degrees C and in lyophilized state compared to other storage conditions used in this study. Lactate dehydrogenase release assay performed on H630 cells indicated that exposure to the venom could result in damage to the cell membrane. |
| 学科主题 | Public, Environmental & Occupational Health ; Toxicology |
| 收录类别 | SCI |
| 原文出处 | 10.1177/0748233711410910 |
| 语种 | 英语 |
| WOS记录号 | WOS:000300222800013 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12216]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Qingdao Coll, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Cuiping,Li Pengcheng,Feng Jinhua,et al. Cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye[J]. TOXICOLOGY AND INDUSTRIAL HEALTH,2012,28(2):186-192. |
| APA | Li Cuiping,Li Pengcheng,Feng Jinhua,Li Rongfeng,&Yu Huahua.(2012).Cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye.TOXICOLOGY AND INDUSTRIAL HEALTH,28(2),186-192. |
| MLA | Li Cuiping,et al."Cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye".TOXICOLOGY AND INDUSTRIAL HEALTH 28.2(2012):186-192. |
入库方式： OAI收割
来源：海洋研究所
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