Development and Linkage Analysis of 104 New Microsatellite Markers for Bay Scallop (Argopecten irradians)
文献类型：期刊论文
| 作者 | Li, Hongjun; Liu, Xiao; Zhang, Guofan |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2012-02-01 |
| 卷号 | 14期号:1页码:1-9 |
| 关键词 | Argopecten irradians Bay scallop Microsatellites Null allele Segregation Linkage map |
| ISSN号 | 1436-2228 |
| 通讯作者 | Liu, X (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 英文摘要 | For genetic analysis and linkage mapping of bay scallop (Argopecten irradians), a set of 120 novel simple sequence repeat markers were developed from microsatellite-enriched libraries and expressed sequence tags. An inter-subspecies hybrid bay scallop family (CC5) of 46 progeny was analyzed as the reference population to confirm polymorphism and test the segregation patterns of these loci. A total of 104 microsatellite markers were polymorphic in the reference family, among which 36 in female, 28 in male, and 40 in both parents, respectively. Linkage analysis allowed mapping these markers to 15 linkage groups, which is close to the haploid chromosome number of bay scallop (n = 16). Analysis of the 40 markers segregating in both parents showed a higher recombination rate in the female parent, with the average of female-to-male recombination ratio of 1.09:1 between linked pairs of markers. When null alleles were considered, there were 17 loci showing segregation distortion at the 5% significance level using the chi-square test. The microsatellite markers developed in this study provide a useful resource for future linkage mapping and quantitative loci analysis in A. irradians. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s10126-011-9383-6 |
| 语种 | 英语 |
| WOS记录号 | WOS:000298882700001 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12219]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hongjun,Liu, Xiao,Zhang, Guofan. Development and Linkage Analysis of 104 New Microsatellite Markers for Bay Scallop (Argopecten irradians)[J]. MARINE BIOTECHNOLOGY,2012,14(1):1-9. |
| APA | Li, Hongjun,Liu, Xiao,&Zhang, Guofan.(2012).Development and Linkage Analysis of 104 New Microsatellite Markers for Bay Scallop (Argopecten irradians).MARINE BIOTECHNOLOGY,14(1),1-9. |
| MLA | Li, Hongjun,et al."Development and Linkage Analysis of 104 New Microsatellite Markers for Bay Scallop (Argopecten irradians)".MARINE BIOTECHNOLOGY 14.1(2012):1-9. |
入库方式： OAI收割
来源：海洋研究所
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