Effect of hormone implantation on cryopreservation of Atlantic halibut (Hippoglossus hippoglossus L.) sperm
文献类型：期刊论文
| 作者 | Ding, Fuhong2; Milley, Joyce E.1; Rommens, Melissa3; Li, Jun4; Lei, Jilin2; Lall, Santosh P.1 |
| 刊名 | CRYOBIOLOGY
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 65期号:1页码:51-55 |
| 关键词 | Atlantic halibut (Hippoglossus hippoglossus L) Hormone (GnRHa) Cryopreservation Spermotocrit Fertilization |
| ISSN号 | 0011-2240 |
| 通讯作者 | Lall, SP (reprint author), Natl Res Council Canada, Inst Marine Biosci, Halifax, NS B3H 3Z1, Canada. |
| 英文摘要 | Hormone implantation is widely applied in halibut (Hippoglossus hippoglossus L) aquaculture to extend the sperm production season of broodstock males. The ability to combine this technique with cryopreservation would increase sperm availability, thereby improving reproduction success and facilitating gene management. In this paper, the cryopreservation ability of sperm from hormone-treated males was examined at three times post-implantation and compared with that of sperm from males that were not hormone-treated. All sperm samples were cryopreserved using the same method. The effectiveness of these techniques was assessed by examining the fertilization rate and motility of thawed sperm. The spermotocrit and concentration of fresh sperm samples were measured to reveal the effect of hormone implantation on sperm characteristics. The reported results indicate that hormone implantation did not affect cryopreservation efficiency. The fertilization rate resulting from thawed sperm of hormone-treated males showed no significant difference from that of untreated males or from fresh sperm. A significant positive relationship was demonstrated between the spermatocrit and concentration of sperm; and a significant decrease of spermatocrit was found in sperm collected from hormone-treated males 14 days post-implantation. No significant linear relationship between spermotocrit and fertilization rate of thawed sperm was shown. Crown Copyright (c) 2012 Published by Elsevier Inc. All rights reserved. |
| 学科主题 | Biology ; Physiology |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.cryobiol.2012.04.001 |
| 语种 | 英语 |
| WOS记录号 | WOS:000305548400008 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12223]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Natl Res Council Canada, Inst Marine Biosci, Halifax, NS B3H 3Z1, Canada 2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 3.Scotian Halibut Ltd, Clarks Harbour, NS B0W 1P0, Canada 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Fuhong,Milley, Joyce E.,Rommens, Melissa,et al. Effect of hormone implantation on cryopreservation of Atlantic halibut (Hippoglossus hippoglossus L.) sperm[J]. CRYOBIOLOGY,2012,65(1):51-55. |
| APA | Ding, Fuhong,Milley, Joyce E.,Rommens, Melissa,Li, Jun,Lei, Jilin,&Lall, Santosh P..(2012).Effect of hormone implantation on cryopreservation of Atlantic halibut (Hippoglossus hippoglossus L.) sperm.CRYOBIOLOGY,65(1),51-55. |
| MLA | Ding, Fuhong,et al."Effect of hormone implantation on cryopreservation of Atlantic halibut (Hippoglossus hippoglossus L.) sperm".CRYOBIOLOGY 65.1(2012):51-55. |
入库方式： OAI收割
来源：海洋研究所
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