Bio-assembling Macro-Scale, Lumenized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion
文献类型：期刊论文
| 作者 | Liu, Ye; Dabrowska, Catherine; Mavousian, Antranik; Strauss, Bernhard3; Meng, Fanlong4 ; Mazzaglia, Corrado5; Ouaras, Karim6; Macintosh, Callum; Terentjev, Eugene7; Lee, Joo-Hyeon
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| 刊名 | ADVANCED SCIENCE
 |
| 出版日期 | 2021 |
| 卷号 | 8期号:9页码:2003332 |
| DOI | 10.1002/advs.202003332 |
| 英文摘要 | Epithelial, stem-cell derived organoids are ideal building blocks for tissue engineering, however, scalable and shape-controlled bio-assembly of epithelial organoids into larger and anatomical structures is yet to be achieved. Here, a robust organoid engineering approach, Multi-Organoid Patterning and Fusion (MOrPF), is presented to assemble individual airway organoids of different sizes into upscaled, scaffold-free airway tubes with predefined shapes. Multi-Organoid Aggregates (MOAs) undergo accelerated fusion in a matrix-depleted, free-floating environment, possess a continuous lumen, and maintain prescribed shapes without an exogenous scaffold interface. MOAs in the floating culture exhibit a well-defined three-stage process of inter-organoid surface integration, luminal material clearance, and lumina connection. The observed shape stability of patterned MOAs is confirmed by theoretical modelling based on organoid morphology and the physical forces involved in organoid fusion. Immunofluorescent characterization shows that fused MOA tubes possess an unstratified epithelium consisting mainly of tracheal basal stem cells. By generating large, shape-controllable organ tubes, MOrPF enables upscaled organoid engineering towards integrated organoid devices and structurally complex organ tubes. |
| 学科主题 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| 源URL | [http://ir.itp.ac.cn/handle/311006/27277]  |
| 专题 | 理论物理研究所_理论物理所1978-2010年知识产出 |
| 作者单位 | 1.Univ Cambridge, Dept Engn, Cambridge CB2 1PZ, England 2.Univ Cambridge, Wellcome MRC Cambridge Stem Cell Inst, Cambridge CB2 0AW, England 3.Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge CB2 3EG, England 4.Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England 5.Chinese Acad Sci, Inst Theoret Phys, CAS Key Lab Theoret Phys, Beijing 100190, Peoples R China 6.Univ Cambridge, MRC Canc Unit, Cambridge CB2 0XZ, England 7.Univ Cambridge, Nanosci Ctr, Cambridge CB30FF, England 8.Univ Cambridge, Cavendish Lab, Cambridge CB3 0HE, England |
| 推荐引用方式 GB/T 7714 | Liu, Ye,Dabrowska, Catherine,Mavousian, Antranik,et al. Bio-assembling Macro-Scale, Lumenized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion[J]. ADVANCED SCIENCE,2021,8(9):2003332. |
| APA | Liu, Ye.,Dabrowska, Catherine.,Mavousian, Antranik.,Strauss, Bernhard.,Meng, Fanlong.,...&Huang, Yan Yan Shery.(2021).Bio-assembling Macro-Scale, Lumenized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion.ADVANCED SCIENCE,8(9),2003332. |
| MLA | Liu, Ye,et al."Bio-assembling Macro-Scale, Lumenized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion".ADVANCED SCIENCE 8.9(2021):2003332. |
入库方式： OAI收割
来源：理论物理研究所
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