A novel mechanism for user-friendly and self-activated microdroplet generation capable of programmable control
文献类型：期刊论文
| 作者 | Jiang, Y. Y.; Du, L.; Li, Y. M.; Mu, Q. Q.; Cui, Z. X.; Zhou, J. ; Wu, W. M.
|
| 刊名 | Analyst
 |
| 出版日期 | 2018 |
| 卷号 | 143期号:16页码:11 |
| 关键词 | polymerase-chain-reaction microfluidic devices chip pcr droplets syringe amplification bubbles pump Chemistry |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c8an00035b |
| 英文摘要 | Unlike conventional approaches that require bulky and expensive pumping equipment, herein, we present a simple method for self-activated microdroplet generation and transport inside a long microchannel. The high gas-pressure in the syringes is used to provide the built-in power of self-priming so that the continuous phase and the dispersed phase are sequentially automated into the generator junction to produce stabilized droplets. The volume ratio between the aqueous and oil phases can be adjusted in a flexible way by accurately controlling the volume of the compressed air in the two syringes, and a novel self-activated micropumping mechanism is introduced to explain this phenomenon. Through the flow rate test inside the microchannels under different conditions, it is found that the flow rate of microdroplets inside the Teflon microchannel is highly stable. As a proof of concept, this novel micropump is applied for a 3D spiral chip for flow through PCR. It is demonstrated that this self-activated micropump is acceptable for droplet-based continuous flow microfluidic PCR with the thermal-cycle controlled by a single thermostatic heater, while the real-time (RT) fluorescence signal is comparable to a commercial qPCR cycler. This self-activated, portable, and controllable droplet generator would extend the droplet-based applications to in-field analysis and facilitate the exploitation of droplet microfluidics by non-technical users. |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/60561]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | Jiang, Y. Y.,Du, L.,Li, Y. M.,et al. A novel mechanism for user-friendly and self-activated microdroplet generation capable of programmable control[J]. Analyst,2018,143(16):11. |
| APA | Jiang, Y. Y..,Du, L..,Li, Y. M..,Mu, Q. Q..,Cui, Z. X..,...&Wu, W. M..(2018).A novel mechanism for user-friendly and self-activated microdroplet generation capable of programmable control.Analyst,143(16),11. |
| MLA | Jiang, Y. Y.,et al."A novel mechanism for user-friendly and self-activated microdroplet generation capable of programmable control".Analyst 143.16(2018):11. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。