Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe
文献类型：期刊论文
| 作者 | Zhou, Jin ; Zhang, Mei-Ling; Zhang, Li; Li, Cui-Xia; Zhao, Hui-Ying; Zhang, You-Lin; Xia, An-Dong; Kong, Xiang-Gui
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| 刊名 | Faguang Xuebao/Chinese Journal of Luminescence
 |
| 出版日期 | 2018 |
| 卷号 | 39期号:8页码:1059-1065 |
| 关键词 | Bioassay Antigens Biosensors Chlorine compounds Diagnosis Immunology Infrared devices Luminescence Silicon wafers Synthesis (chemical) |
| ISSN号 | 10007032 |
| DOI | 10.3788/fgxb20183908.1059 |
| 英文摘要 | Uniformed NaYF4:Yb3+,Tm3+ nanoparticles were synthesized through solvent thermal method in chloride. PAA-based ligand replace strategies were carried out to introduce free carboxylic acid groups which allowed further conjugation with NH2-containing human IgG. The silicon wafer was modified with rabbit anti-goat IgG by using protein G as "bridge", which contributed to eliminate structural or conformational changes of IgG. Owing to the specific antibody recognition to the antigenic, a simple, rapid, efficient and sensitive immunoassay method was developed. The experimental results demonstrate that there is a very good linear correlation between the upconversion luminescence intensity and the amount of goat anti-human IgG in the range of 5~400 nmol/L. The detection limit is as low as 1.82 nmol/L. Our developed detection system shows excellent detection specificity and sensitivity, which provides a new detection method and platform with wide linear range and high sensitivity for diagnosis of major clinical diseases in the future. 2018, Science Press. All right reserved. |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/60820]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, Jin,Zhang, Mei-Ling,Zhang, Li,et al. Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe[J]. Faguang Xuebao/Chinese Journal of Luminescence,2018,39(8):1059-1065. |
| APA | Zhou, Jin.,Zhang, Mei-Ling.,Zhang, Li.,Li, Cui-Xia.,Zhao, Hui-Ying.,...&Kong, Xiang-Gui.(2018).Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe.Faguang Xuebao/Chinese Journal of Luminescence,39(8),1059-1065. |
| MLA | Zhou, Jin,et al."Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe".Faguang Xuebao/Chinese Journal of Luminescence 39.8(2018):1059-1065. |
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