Improvement in focusing accuracy of DNA sequencing microscope with multi-position laser differential confocal autofocus method
文献类型：期刊论文
| 作者 | Zhang, X. ; Zeng, F.; Li, Y.; Qiao, Y. F.
|
| 刊名 | Optics Express
 |
| 出版日期 | 2018 |
| 卷号 | 26期号:2页码:895-904 |
| 关键词 | focal-length measurement biological nanopore radius measurement system Optics |
| ISSN号 | 1094-4087 |
| DOI | 10.1364/oe.26.000887 |
| 英文摘要 | High focusing accuracy in microscopes could improve the imaging quality to reduce the error rate in DNA sequencing. We propose a new feedback method to improve the focusing condition to a very high accuracy. A reference laser reflected by the sample is detected by two or more sensors around the confocal point. After acquiring the signals from the out-of-focus positions, online data processing is implemented to provide feedbacks for real-time focus-plane locking on the sample surface. This method provides an accuracy better than 1/10 of the objective depth-of-focus. To balance optical aberrations, a specific optical feedback system should be designed, with athermal design considerations to adapt DNA sequencing work to temperature fluctuations. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/61032]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, X.,Zeng, F.,Li, Y.,et al. Improvement in focusing accuracy of DNA sequencing microscope with multi-position laser differential confocal autofocus method[J]. Optics Express,2018,26(2):895-904. |
| APA | Zhang, X.,Zeng, F.,Li, Y.,&Qiao, Y. F..(2018).Improvement in focusing accuracy of DNA sequencing microscope with multi-position laser differential confocal autofocus method.Optics Express,26(2),895-904. |
| MLA | Zhang, X.,et al."Improvement in focusing accuracy of DNA sequencing microscope with multi-position laser differential confocal autofocus method".Optics Express 26.2(2018):895-904. |
入库方式： OAI收割
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