Passive Micropump for Highly Stable, Long-Termed, and Large Volume of Droplet GenerationTransport Inside 3D Microchannels Capable of Surfactant-Free and Droplet-Based Thermocycled Reverse Tran
文献类型：期刊论文
| 作者 | Li, Y. M.; Jiang, Y. Y.; Wang, K. N.; Wu, W. M. |
| 刊名 | Analytical Chemistry
 |
| 出版日期 | 2018 |
| 卷号 | 90期号:20页码:11925-11932 |
| 关键词 | continuous-flow pcr microfluidic control sample injection syringe pump device chip pdms miniaturization amplification microdevice Chemistry |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b02271 |
| 英文摘要 | It is still one key challenge for traditional passive micropumps (e.g., surface tension micropump, hydrostatic micropump, enzymatic micropump, degassed-polydimethylsiloxane (PDMS) micropump, etc.) to transport a large volume of two-phased fluid for a long period. Herein we propose a user-friendly and passive approach to realize the microdroplet generation by waiving expensive or complex equipment. The automation principle is systemically studied in this paper. It is affirmed that this micropump can continuously transport over 2000 mu L of two-phased aqueous/oil microdroplets over a 4 m long 3D microchannel for 8 h. In addition, variations in flow rate are little within each hour-period, and the evaporation bubbles can be well suppressed under high temperature (95 degrees C). As a proof of this concept, the novel micropump is applied to droplet-based continuous flow real-time polymerase chain reactions (PCRs), which only require several disposable syringes for oil/aqueous-phase storage, two 34 gauge needles for droplet generation, a Teflon tube for PCR amplification, and a single thermostatic heater for the thermal cycle. The results suggest this droplet generation method is acceptable for a house-made setup of microfluidic PCRs. Besides, the amplification efficiency of the droplet-based microcontinuous flow PCRs here is much higher than the plug-based microcontinuous flow PCRs in our previous work and reaches 91% of the commercial qPCR thermocycler for the target gene of Rubella virus (Ruby). Without expensive microfabrication instruments, this novel method is more accessible to nonprofessionals than previous reports and would extend the droplet-based applications to in-field and real-time analysis. |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/61100]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | Li, Y. M.,Jiang, Y. Y.,Wang, K. N.,et al. Passive Micropump for Highly Stable, Long-Termed, and Large Volume of Droplet GenerationTransport Inside 3D Microchannels Capable of Surfactant-Free and Droplet-Based Thermocycled Reverse Tran[J]. Analytical Chemistry,2018,90(20):11925-11932. |
| APA | Li, Y. M.,Jiang, Y. Y.,Wang, K. N.,&Wu, W. M..(2018).Passive Micropump for Highly Stable, Long-Termed, and Large Volume of Droplet GenerationTransport Inside 3D Microchannels Capable of Surfactant-Free and Droplet-Based Thermocycled Reverse Tran.Analytical Chemistry,90(20),11925-11932. |
| MLA | Li, Y. M.,et al."Passive Micropump for Highly Stable, Long-Termed, and Large Volume of Droplet GenerationTransport Inside 3D Microchannels Capable of Surfactant-Free and Droplet-Based Thermocycled Reverse Tran".Analytical Chemistry 90.20(2018):11925-11932. |
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