Split aptamer-based detection of adenosine triphosphate using surface enhanced Raman spectroscopy and two kinds of gold nanoparticles
文献类型：期刊论文
| 作者 | C.Y.Zhou; Z.Yu; W.L.Yu; H.W.Liu; H.Zhang; C.L.Guo |
| 刊名 | Microchimica Acta
 |
| 出版日期 | 2019 |
| 卷号 | 186期号:4页码:9 |
| 关键词 | Sandwich nanostructure,Biosensor,Graphene oxide,SERS,Human serum,Aptasensor,Diagnostics,graphene oxide,scattering,probe,atp,Chemistry |
| ISSN号 | 0026-3672 |
| DOI | 10.1007/s00604-019-3356-2 |
| 英文摘要 | An ultrasensitive and highly selective method is described for the determination of adenosine triphosphate (ATP) via surface-enhanced Raman scattering (SERS). Two split aptamers are used for specific recognition of ATP. They were attached to two SERS substrates. The first was placed on a nanolayer of gold nanoparticle-decorated graphene oxide (GO/Au3), and the other on gold nanoparticles (Au2). When ATP is introduced, it will interact with the split aptamers on the gold nanostructures to form a sandwich structure that brings the GO/Au3 nanolayer and the Au2 nanoparticle in close proximity. Consequently, the SERS signal, best measured at 1072cm(-1), is strongly enhanced. The sandwich structure also displays good water solubility and stability. Under optimized conditions, the SERS signal increases in the 10 pM-10nM ATP concentration range, and the limit of detection (LOD) is 0.85 pM. The method was applied to the determination of ATP in spiked human serum, and the LODs in serum and buffer are comparable. In our perception, the method has a wide scope in that numerous other aptamers may be used. This may result in a variety of other highly sensitive aptasensors for use in in-vitro diagnostics. |
| 语种 | 英语 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/62735]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | C.Y.Zhou,Z.Yu,W.L.Yu,et al. Split aptamer-based detection of adenosine triphosphate using surface enhanced Raman spectroscopy and two kinds of gold nanoparticles[J]. Microchimica Acta,2019,186(4):9. |
| APA | C.Y.Zhou,Z.Yu,W.L.Yu,H.W.Liu,H.Zhang,&C.L.Guo.(2019).Split aptamer-based detection of adenosine triphosphate using surface enhanced Raman spectroscopy and two kinds of gold nanoparticles.Microchimica Acta,186(4),9. |
| MLA | C.Y.Zhou,et al."Split aptamer-based detection of adenosine triphosphate using surface enhanced Raman spectroscopy and two kinds of gold nanoparticles".Microchimica Acta 186.4(2019):9. |
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